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Minerva Endocrinology 2022 September;47(3):304-13

DOI: 10.23736/S2724-6507.20.03257-5

Copyright © 2020 EDIZIONI MINERVA MEDICA

language: English

Inflammation is associated with impairment of oxidative status, carbohydrate and lipid metabolism in type 2 diabetes complicated by non-alcoholic fatty liver disease

Sergey S. POPOV 1, Evgenii D. KRYL’SKII 2 , Konstantin K. SHULGIN 2, Ekaterina A. RASKINA 1, Tatyana N. POPOVA 2, Alexander N. PASHKOV 3, Galina N. KUPTSOVA 1

1 Department of Hospital Therapy and Endocrinology, Voronezh State Medical University Named After N.N. Burdenko, Voronezh, Russia; 2 Department of Medical Biochemistry and Microbiology, Voronezh State University, Voronezh, Russia; 3 Department of Biology, Voronezh State Medical University named after N. N. Burdenko, Voronezh, Russia



BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is accompanied by inflammation and impairment of the lipid metabolism. In addition, NAFLD is one of the major complications of type 2 diabetes associated with oxidative stress. Based on this, we evaluated the tumor necrosis factor alpha (TNF-α), nuclear factor κB (NF-κB), oxidative status rates, and analyzed its correlation with carbohydrate and lipid metabolism in patients with NAFLD and type 2 diabetes.
METHODS: A case-control study included 63 participants with NAFLD developing in patients with type 2 diabetes, and 65 healthy volunteers with a normal complete blood count and blood biochemical profile. The following parameters and states were assessed during the study: glycaemia, insulin resistance, lipid levels, liver tests, intensity of free radical induced oxidation, antioxidant enzymes, TNF-α and NF-κB level.
RESULTS: Free radical induced oxidation was significantly elevated (P<0.001), total antioxidant activity was significantly decreased (P<0.001) and associated with insulin resistance (P=0.019) and lipid metabolism shifts (P<0.05) in patients with NAFLD and type 2 diabetes. Such patients had showed impaired functioning of antioxidant system (P<0.001), inhibition of NADPH-generating enzymes activity (P<0.001), increased levels of TNF-α (P<0.001) and NF-κB (P=0.019) correlated with the severity of hyperglycemia (P<0.05), concentration of reduced glutathione (P=0.005) and total cholesterol (P=0.016).
CONCLUSIONS: The increase of free radical induced oxidation, TNF-α and NF-κB levels, and depletion of the antioxidant system seems to be the key factors of the development of NAFLD in patients with type 2 diabetes.


KEY WORDS: Diabetes mellitus, type 2; Non-alcoholic fatty liver disease; Inflammation, Oxidative stress

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