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Minerva Endocrinologica 2017 March;42(1):30-40

DOI: 10.23736/S0391-1977.16.02351-8

Copyright © 2015 EDIZIONI MINERVA MEDICA

language: English

Isolation and expansion of cytokeratin positive progenitor cells from adult murine pancreatic ducts expressing Pdx-1, Nestin, Sox9, MafA and hepatic nuclear factors

Ulrich GRABMAIER 1, 2, Isabelle ROTTSTEGGE 1, Monika OFFERS 1, Tilmann ZIEGLER 2, 3, Christoph BRENNER 2, Bruno C. HUBER 2, Jochen SEISSLER 1

1 IV Medical Department, Ludwig Maximilians University, Munich, Germany; 2 I Medical Department, Ludwig Maximilians University, Munich, Germany; 3 Department of Molecular Medicine, Max Planck Institute of Biochemistry, Munich, Germany


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BACKGROUND: Recent studies suggest that stem cells may represent a putative source for the generation of beta cells. However, the identity and characteristics of stem cells from adult pancreas and conditions for their large scale expansion are still poorly defined.
METHODS: DPC were isolated from adult pancreatic ducts of C57Bl/6 mice. Expression profile was investigated by PCR, FACS and immunohistochemistry.
RESULTS: DPC express a panel of stem cell associated markers such as Pdx-1, cytokeratin-19 (CK19), nestin, Sox9 together with the transcription factor MafA and hepatic nuclear factors HNF1β, HNF3β, HNF4α und HNF6. This gene expression profile is suggesting that DPC might be a promising tool for endocrine differentiation. After stimulation with picolinic acid and hypoxia, DPC expressed the endocrine differentiation marker Ngn3. Nevertheless, insulin production was not observed.
CONCLUSIONS: We here describe a protocol for the isolation end expansion of murine pancreatic ductal progenitor cells (DPC) displaying high self-renewal, spheroid- and colony-forming capacity. Further studies are required to elucidate the conditions for differentiation into mature pancreatic endocrine cell lineages.


KEY WORDS: Cell hypoxia - Mouse Mafa protein - Mouse Neurog3 protein - Stem cells - Pancreatic and duodenal homeobox 1 protein

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