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Minerva Endocrinologica 2010 June;35(2):37-46


language: English, Italian

NPR-B, the C-type natriuretic peptide specific receptor, is the predominant biological receptor in mouse and pig myocardial tissue

Del Ry S. 1, Cabiati M. 1, Lionetti V. 2, Giannessi D. 1

1 CNR Institute of Clinical Physiology, Laboratory of Cardiovascular Biochemistry,Pisa, Italy; 2 Sector of Medicine, Scuola Superiore Sant’Anna Pisa, Italy


AIM: The increased myocardial production and the elevated plasma concentrations of C-type natriuretic peptide (CNP) in heart failure patients suggest its involvement in pathophysiological cardiac remodeling. The cardiovascular action of CNP seems to be mainly mediated by natriuretic peptide receptor (NPR)-B but the importance of CNP/NPR-B signaling in heart is not yet well characterized. The aim of this study was to assess the cardiac mRNA expression of CNP and NPR-B together with those of BNP and NPR-A in order to evaluate the relative importance of these peptides and of their receptors in cardiovascular system.
METHODS: The expression of mRNA coding for CNP, NPR-B, BNP and NPR-A was investigated in myocardial tissue (BALB/c mice, N=5) by use of RT-PCR. NPR-A and NPR-B expression were also evaluated in left ventricle of male adult minipigs without (N=5) and with pacing-induced heart failure (HF, N=5).
RESULTS: The proposed method allowed to detect the expression of mRNA coding for CNP and NPR-B in myocardial tissue confirming the presence of these effectors in the heart. These data also indicate that CNP mRNA expression is lower with respect to that of BNP (CNP/GAPDH= 0.117±0.035 vs. BNP/GAPDH=0.247±0.066) and that NPR-B is the predominant subtype receptor in the heart (Mouse: NPR-A/GAPDH=0.244± 0.028; NPR-B/GAPDH=0.657±0.022; p=0.0008; Pig: NPR-A/GAPDH=3.06±1.75, NPR-B/GAPDH= 14.3±3.6, p=0.0028; HF Pig: NPR-A/GAPDH= 4.29±0.93, NPR-B/GAPDH=7.9±1.1, p=0.0043).
CONCLUSION: In the present study, we provided the first evidence of a higher mRNA expression in cardiac tissue of NPR-B with respect to NPR-A indicating that CNP specific receptor (NPR-B) is the predominant biological receptor in mouse and pig myocardial tissue.

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