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Minerva Biotecnologica 2017 September;29(3):139-44

DOI: 10.23736/S1120-4826.17.02242-X

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Methylation status and expression of SOX17 gene in endometrioid carcinoma tissues

Yu-Mei LIAO , Ying SHI, Xiao-Shuang REN, Wu-Liang WANG, Xiao-Li GU, Yang SONG

Department of Obstetrics and Gynecology, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, China


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BACKGROUND: The aim of this study was to investigate methylation status and expression of the SOX17 gene in endometrioid carcinoma (EC) tissues.
METHODS: Forty-two specimens of EC tissues and 10 specimens of normal proliferative phase endometrium were collected in the Second Affiliated Hospital of Zhengzhou University, while 10 specimens of peripheral blood were obtained from healthy women as controls. The methylation status of the SOX17 gene in endometrial tissues was detected using methylation-specific PCR (MSP). The SOX17 gene expression in EC tissues was detected by immunohistochemical SP method.
RESULTS: 1) Methylation of the SOX17 gene can be detected in 35 specimens (83.3%) of EC. It was in complete methylation in 23 specimens, and was in partial methylation in 12 specimens. Methylation was not detected in 10 specimens of normal endometrial tissues. The difference in methylation status between EC tissues and normal endometrium was statistically significant (P<0.05). 2) The methylation status of the SOX17 gene was correlated with the degree of tumor differentiation (P=0.01) and the depth of muscular invasion (P=0.023). 3) The positive expression rate of the SOX17 protein was 38.09% in 42 specimens of EC. The difference in SOX17 protein expression between EC specimens and normal endometrium was statistically significant (X2=12.381, P<0.05).
CONCLUSIONS: 1) The CpG islands of the SOX17 gene in EC tissues were highly methylated and the level of protein expression was significantly decreased, suggesting that the SOX17 gene may act as a cancer suppressor gene in the occurrence of EC. 2) The methylation status of the SOX17 gene was correlated with the degree of tumor differentiation and depth of muscular invasion.


KEY WORDS: Carcinoma, endometrioid - Methylation - Epigenesis, genetic

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