ORIGINAL ARTICLE   

Minerva Biotechnology and Biomolecular Research 2022 September;34(3):131-6

DOI: 10.23736/S2724-542X.22.02905-4

Copyright © 2022 EDIZIONI MINERVA MEDICA

language: English

Prevalence of Non-O157 Escherichia coli serotypes isolated from the stool of under five years old children presenting with diarrhea in Lagos, Nigeria

Tolulope F. JOLAIYA ¹, Sascha D. BRAUN ^{2, 3}, Abraham AJAYI ¹, Akitoye O. COKER ⁴, Ralf EHRICHT ^{2, 3, 5}, Stefan MONECKE ^{2, 3, 6}, Rinaldo PELLICANO ⁷, Stella I. SMITH ¹ ✉

¹ Department of Molecular Biology and Biotechnology, Nigerian Institute of Medical Research, Lagos, Nigeria; ² Leibniz Institute of Photonic Technology (IPHT), Jena, Germany; ³ InfectoGnostics Research Campus, Jena, Germany; ⁴ Babcock University, Ogun, Nigeria; ⁵ Institute of Physical Chemistry, Friedrich-Schiller University, Jena, Germany; ⁶ Institute for Medical Microbiology and Virology, Dresden University Hospital, Dresden, Germany; ⁷ Unit of Gastroenterology, Molinette Hospital, Turin, Italy

BACKGROUND: Escherichia coli (E. coli) serotypes have been implicated as etiological agents in the pathogenesis of diarrhea in children. The aim of this study was to investigate the prevalence, virulence and antibiotic resistance profile of serotypes of E. coli in children between 0 and 5 years with diarrhea in Lagos, Nigeria, the most populous country in Africa.
METHODS: Stool samples of 200 children with diarrhea and 50 apparently healthy controls attending public health facilities were collected for a duration of 5 months. E. coli strains were isolated using standard microbiological techniques and genoserotyped by microarray. Antimicrobial susceptibility testing was done using the disc diffusion method and virulence genes including stx1, stx2 and eaeA were assayed by polymerase chain reaction (PCR).
RESULTS: Fifty non-O157 E. coli serotypes were isolated from 250 stool samples collected. Forty two percent of these were obtained from children aged 11-20 months. A significant number (P<0.0173) of the isolates were recovered from the stool of children with diarrhea. Antimicrobial susceptibility patterns revealed that there was 70%, 80% and 84% resistance to gentamicin, amoxicillin plus clavulanic acid and ampicillin, respectively. Ninety-eight percent of the isolates were sensitive to ceftazidime, ciprofloxacin and ofloxacin. The genes stx1, stx2 and eaeA were detected in only 4 (8%) of the isolates.
CONCLUSIONS: Our findings show a high frequency of non-O157 E. coli serotypes in the stool of children with diarrhea in Lagos, Nigeria.

KEY WORDS: Child; Diarrhea; Escherichia coli; Serotyping; Microarray analysis; Shiga toxin