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Minerva Biotechnology and Biomolecular Research 2022 September;34(3):122-30

DOI: 10.23736/S2724-542X.21.02866-2

Copyright © 2021 EDIZIONI MINERVA MEDICA

language: English

Isolation and screening of alpha-galactosidase-producing probiotics with anti-flatulence potential

Aboozar KAZEMI 1, 2, Shiva HEMMATI 1, 2, Mohammad H. MOROWVAT 1, 2, Ahmad GHOLAMI 1, 2, Younes GHASEMI 1, 2

1 Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 2 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran



BACKGROUND: Probiotics are nonpathogenic living microorganisms with excellent health and well-being benefits to their host. In this work, the isolation, identification, and probiotic characterization of alpha-galactosidase (EC 3.2.1.22) producing bacteria with antiflatulence potential were studied in human fecal microbiota.
METHODS: Samples were cultured in acidified De Man, Rogosa and Sharpe agar (MRS) broth to select acid-tolerant lactobacilli bacteria. Isolates were then screened based on forming yellow colonies on MRS plates containing 0.01% bromocresol purple to select the alpha-galactosidase producing bacteria. The alpha-galactosidase activity was assayed according to the DNS method. For probiotic characterization, tolerance to acid and bile salts, antibiotics sensitivity, pathogen inhibition, cytotoxicity, adherence to Caco-2 cells, aggregation, and hydrophobicity of selected isolates were evaluated.
RESULTS: A total of 320 samples were examined to isolate probiotics. About 121 colonies were grown, which were rod shape, gram-positive, and catalase-negative. Among them, 21 isolates produced alpha-galactosidase and showed probiotic properties. Lactobacillus acidophilus BCCS A49 and Lactobacillus acidophilus BCCS A50 showed potential probiotic characteristics and produced a high yield of alpha-galactosidase (0.6±0.05 U/mL and 0.86±0.04 U/mL, respectively).
CONCLUSIONS: These two strains could be regarded as promising candidates hydrolyzing alpha-galactosyl moieties and reducing gastrointestinal gas in a safe approach which, of course, requires further studies.


KEY WORDS: Probiotics; Lactobacillus; Alpha-galactosidase; Flatulence

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