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Minerva Biotecnologica 2019 June;31(2):68-73

DOI: 10.23736/S1120-4826.19.02548-5


language: English

Effect of probiotic on serum cytokines and matrix metalloproteinases profiles during monoiodoacetate-induced osteoarthritis in rats

Oleksandr KOROTKYI 1, Andrii VOVK 1, Tetiana GALENOVA 1, Tetyana VOVK 1, Kateryna DVORSCHENKO 1, Francesco LUZZA 2, Ludovico ABENAVOLI 2, Nazarii KOBYLIAK 3, Tetyana FALALYEYEVA 4 , Liudmyla OSTAPCHENKO 1

1 Department of Biochemistry, Educational and Scientific Center “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine; 2 Department of Health Sciences, Magna Græcia University, Catanzaro, Italy; 3 Department of Endocrinology, Bogomolets National Medical University, Kyiv, Ukraine; 4 Department of Biomedicine, Educational and Scientific Center “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

BACKGROUND: Cytokines (CKs) and matrix metalloproteinases (MMPs) play a major role in the pathogenesis of osteoarthritis (OA). The recent studies showed the effect of probiotics (PBs) on inflammatory processes during OA, but available results are limited and conflicted. In this study, we investigated the effect of alive probiotic on the levels of CKs (interleukins [ILs] IL-1β, IL-4, IL-10, IL-12 p40, tumor necrosis factor [TNF]-α, interferon [IFN]-γ) and MMPs (MMP-1, MMP-2, MMP-3, MMP-8) in rats with monoiodoacetate-induced OA.
METHODS: We used a single injection of monoiodoacetate through the infrapatellar ligament of nonlinear male rats to start OA model (1st day of the experiment). Therapeutic groups got an intragastric feeding of PB composition (“Symbiter” O.D. Prolisok, Kyiv, Ukraine) from 8th to 21st. Sampling (serum, knee joint) was provided on the 30th day. The pathophysiological aspects of OA was described by histology assessment of knee cartilage. The levels of CKs and MMPs were measured in serum by enzyme-linked immunosorbent assay.
RESULTS: OA model caused a significant increasing the levels of pro-inflammatory CKs (IL-1β, TNF-α, IFN-γ), and all studied MMPs, while IL-4 and IL-10 were decreased, comparing to group without OA (except IL12 p40, no significance changes in all groups). An application of the PB had positive effect on the levels of CKs and MMPs.
CONCLUSIONS: The use of the PBs has anti-inflammatory effect during experimental OA. Thus, action of PB is promising and further investigations are waited with interest.

KEY WORDS: Inflammation; Models, animal; Osteoarthritis; Cytokines; Matrix metalloproteinases; Probiotics

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