ORIGINAL ARTICLES   

Minerva Biotecnologica 2013 December;25(4):219-25

Copyright © 2013 EDIZIONI MINERVA MEDICA

language: English

Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis

Mazmira M. M. M. ^{1, 2}, Ramlah S. A. A. ¹, Rosfarizan M. ², Ling T. C. ³, Ariff A. B. ² ✉

¹ Biological Research Division, Malaysian Palm Oil Board Kajang, Selangor, Malaysia; ² Department of Bioprocess Technology Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, Malaysia; ³ Institute of Biological Sciences, Faculty of Science Building, University of Malaya, Kuala Lumpur, Malaysia

The relationship between intracellular and extracellular total carbon, total nitrogen and carbon to nitrogen ratio on Bacillus thuringiensis MPK13 growth, sporulation rate and d-endotoxin synthesis were carried out in shake flask using different types of carbon (glucose, galactose, sucrose, lactose and maltose) and nitrogen (yeast extract) sources. The highest initial intracellular TC (5.15 g/L) and intracellular TN (1.45 g/L) were recorded in medium containing glucose. On the other hand, the highest initial extracellular TC (34.54 g/L) and TN (7.61 g/L) were recorded in medium containing maltose and lactose, respectively. At 48 h of cultivation, the highest final intracellular TC (11.96 g/L) and intracellular TN (3.26 g/L) were also recorded in medium containing glucose. The highest final extracellular TC (27.85 g/L) and TN (7.27 g/L) were recorded in medium containing galactose which showed the lowest growth and sporulation. The presence of d-endotoxin was only detected at 48 h of cultivation using medium containing glucose. The result indicated that high initial intracellular TC and TN values during the cultivation anticipated in high cell growth, sporulation and d-endotoxin production and may be used for indirect measurement of cultivation performance for B. thuringiensis.