MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002 

Minerva Biotecnologica 2002 December;14(3-4):265-7

Copyright © 2003 EDIZIONI MINERVA MEDICA

language: English

Ligase detection reaction and universal array as a tool to detect grapevine infecting phytoplasmas

Frosini A. ¹, Casati P. ⁴, Bianco P. A. ⁴, Bordoni R. ², Consolandi C. ¹, Castiglioni B. ³, Mezzelani A. ², Rizzi E. ², Battaglia C. ¹, Belli G. ⁴, Rossi Bernardi L. ¹, De Bellis G. ²

¹ Department of Biomedical Technologies and Sciences, University of Milan, LITA, Segrate (Milano), Italy; ² Institute of Biomedical Technologies, National Council for Scientific Research, Milan, Italy; ³ Institute of Agricultural Biotechnology and Biology, National Council for Scientific Research, Milan, Italy; ⁴ Institute of Plant Pathology, University of Milan, Milan, Italy

Phy­to­plas­mas are ­unculturable wall ­less bac­te­ria ­involved in ­many ­plant dis­eas­es; in par­tic­u­lar Fla­ves­cence ­dorée rep­re­sents a ­severe ­threat to vit­i­cul­ture, ­because of its epidemic behavior in field. ­Here we ­present the appli­ca­tion of the LDR tech­nique, asso­ciat­ed ­with hybrid­isa­tion on uni­ver­sal ­arrays, as a ­quick and reli­able ­tool for ­plant qua­ran­tine pro­ce­dures and man­da­to­ry meas­ures, in ­order to dis­tin­guish ­between dif­fer­ent ­phytoplasmas’ ­induced infec­tions, com­pared to clas­si­cal pro­to­cols in use, par­tic­u­lar­ly PCR - ­RFLP ­assays.