FLOW CYTOMETRY IN IMMUNOLOGY AND HEMATOLOGY 

Minerva Biotecnologica 1999 June;11(2):153-6

Copyright © 1999 EDIZIONI MINERVA MEDICA

language: English

Cell sorting using flow cytometry. Old instruments for new applications

Melioli G., Pasquetti W., Verlino C., Semino C.

Citometry Unit, Advanced Biotechnology Center, National Institute for Cancer Research, Genova, Italy

The use of ­flow cytom­etry and ­cell ­sorting ­started in ­research and clin­ical inves­ti­ga­tion labor­a­to­ries in 1980. At ­present, ­cell ­sorting has a ­number of ­classic indi­ca­tions, in par­tic­ular in the ­field of immu­nology and hem­a­tology. In addi­tion, new appli­ca­tions, in the ­field of bio­tech­nology (­such as pur­ifi­ca­tion of ­extremely ­rare pop­u­la­tions as ­well as ­multi-par­a­metric sep­ar­a­tion on the ­basis of ­both phys­ical and phe­no­typic param­e­ters) ­have ­been devel­oped ­recently. The tech­nical ­approach for ­these new ­sorting strat­e­gies is cru­cial in ­order to ­obtain reli­able and repro­du­cible ­results.