ORIGINAL ARTICLE   

Minerva Biotecnologica 2018 December;30(4):102-7

DOI: 10.23736/S1120-4826.18.02479-5

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Distribution of Helicobacter pylori genotypes in various sites of the hepatobiliary system

Guzel ISAEVA ^{1, 2} ✉

¹ Kazan Research Institute of Epidemiology and Microbiology, Kazan, Russia; ² Kazan State Medical University, Kazan, Russia

BACKGROUND: The aim of this study was to evaluate the distribution of Helicobacter pylori (H. pylori) genotypes in various sites of the hepatobiliary system.
METHODS: Ninety-five patients with diseases of the hepatobiliary system by polymerasechain reaction (PCR) for the presence of H. pylori were examined: 42 patients with chronic non-calculous cholecystitis (men -17, women -25, mean age 42.6±4.8) 42 patients with chronic calculous cholecystitis (men -7, women - 35, average age 57.1±3.6) and 11 patients with cirrhosis of the liver (male - 7, female - 4). The control group consisted of 30 patients without lesion of the hepatobiliary system (men - 13, women - 17, average age 42.9±3.4).
RESULTS: As a result of testing liver biopsies, bile ducts and gall bladder 95 patients with DNA, the bacterium was detected in 31 patients (32.6%). PCR results for the presence of the H. pylori ureC gene in the control group (N.=30) were negative. The incidence of H. pylori was 50% in noncalculous cholecystitis, 4.76% in calculous cholecystitis, and 72.7% in liver cirrhosis. A comparative study of genotypes of H. pylori isolates were conducted from different habitats of the same patients. As a result, it was found that H. pylori genotypes obtained from different organs of the hepatobiliary system are not identical in some cases. The obtained results indicate that in the colonization of the hepatobiliary tract, H. pyloriureC - positive, cagА, babA2 - negative, vacA - positive strains can be pathogenetic, but differing in the predominance of the m2 allelic variant, which has an insignificant level of toxic activity.
CONCLUSIONS: Detection of differences in H. pylori genotypes isolated from the stomach, bile ducts and liver from a patient with cirrhosis may indicate the presence of selective colonization of different biotopes by different H. pylori subtypes.

KEY WORDS: Helicobacter pylori - Liver cirrhosis - Genotype