PHYSIOLOGICAL AREA   

Medicina dello Sport 2016 June;69(2):173-83

Copyright © 2016 EDIZIONI MINERVA MEDICA

language: English, Italian

Apoptosis response to different rest periods after resistance exercise in athletes

Hassan FARAJI ¹, Rahman RAHIMI ², Dariush SHEIKHOLESLAMI VATANI ², Afshar JAFARI ³

¹ Department of Physical Education and Sports Science, Mariwan Branch, Islamic Azad University, Mariwan, Iran; ² Department of Physical Education and Sports Science, University of Kurdistan, Sanandaj, Iran; ³ Faculty of Physical Education and Sports Science, University of Tabriz, Tabriz, Iran

BACKGROUND: The purpose of this study was to determine the effect of different rest periods on p53, caspase-9; markers of intrinsic apoptosis, and IGF-1 as an anti-apoptotic biomarker after resistance exercise (RE).
METHODS: Ten athlete men (age 19-26 years and BMI 20-25 kg.m-2) volunteered to participate in this study. Subjects performed two RE protocols on two separate sessions that include 4 sets of 6 exercises at 85% of 1 repetition maximum (1RM) until failure with 1 or 3 minutes rest between sets in randomized crossover manner. Blood samples of p53, caspase-9 and IGF-1 were examined at pre-exercise (Pre), immediately post (Post), 3h post (3h Post) and 24h post (24h Post) both the RE protocols.
RESULTS: Serum IGF-1 concentration was significantly higher post RE for both conditions, compared with their baseline (P<0.05). There was a significant increase (OP<0.05) in post RE caspase-9 concentration only in the 1 min rest condition. Also, there were no significant differences in the concentration of p53 during recovery time after both RE protocols in comparison to pre-exercise (P>0.05). However, there were no significant differences between both RE group (1 min or 3 min rest between sets) values for serum p53, caspase-9 and IGF-1 concentrations in all phase of post RE.
CONCLUSIONS: Although caspase-9 increase significantly just after resting 1 minute, our findings indicate that different rest periods between RE sets do not affect biomarkers of apoptosis including p53 and caspase-9 as well as IGF-1 as anti-apoptotic biomarker in resistance trained athletes.