ORIGINAL ARTICLES   

International Angiology 2004 June;23(2):122-7

Copyright © 2004 EDIZIONI MINERVA MEDICA

language: English

White blood cell and endothelial cell response to endovascular procedures in the leg

Danielsson P. ¹, Danielsson G. ¹, Truedsson L. ², Norgren L. ¹

¹ Department of Vascular Diseases, University Hospital MAS, Malmö, Sweden 2 Department of Microbiology, Immunology and Glycobiology, University Hospital, Lund, Sweden

Aim. The long­term paten­cy of endo­vas­cu­lar pro­ce­dures is part­ly depend­ing on resten­o­sis by inti­mal hyper­pla­sia, a pro­cess depend­ing on inflam­ma­to­ry mech­a­nisms resem­bling the mech­a­nisms of nor­mal ­repair and the pro­cess of ath­ero­scler­o­sis. The aim of this study was to char­ac­ter­ize the inflam­ma­to­ry ­response of white blood cells (WBC) and endo­the­lial cells fol­low­ing endo­vas­cu­lar pro­ce­dures in the leg.
Meth­ods. ­Venous blood sam­ples were drawn from a cubi­tal vein ­before and 2 hours after inter­ven­tion in 19 ­patients suf­fer­ing from periph­er­al arte­ri­al occlu­sive dis­ease (PAOD). Flow cytom­e­try was used to iden­ti­fy and deter­mine the con­cen­tra­tions of WBC and plate­lets and to meas­ure CD11b/CD18 on WBC and CD41 on plate­lets. Sol­u­ble endo­the­lial mark­ers (sICAM-1, sE-selec­tin, sP-selec­tin and sVCAM-1) were meas­ured by ELISA tech­nique.
­Results. WBC were down­reg­u­lat­ed fol­low­ing endo­vas­cu­lar pro­ce­dures. The endo­the­lial cell ­response was lim­it­ed and only down­reg­u­la­tion of sP-selec­tin ­reached sig­nif­i­cant lev­els. The ­results were more evi­dent in the group of ­patients with crit­i­cal limb ische­mia (CLI) com­pared to ­patients with inter­mit­tent claud­i­ca­tion (IC).
Con­clu­sion. Endo­vas­cu­lar pro­ce­dures in the leg evoke only a lim­it­ed ­response which is depend­ing on the ­degree of ische­mia and the mag­ni­tude of the inter­ven­tion­al pro­ce­dure.