ORIGINAL ARTICLES   

International Angiology 1999 December;18(4):320-6

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English

Sodium/hydrogen ion exchange inhibitor ameliorates ischaemia-reperfusion injuries in the rat hind limb

Hamada Y., Ishikawa S., Kanda T., Imai S., Nagai R., Matsui K., Ohashi N., Morishita Y.

From the Second Department of Surgery, Department of Laboratory Medicine, Second Department of Internal Medicine, Gunma University School of Medicine, Gunma, Japan Sumitomo Pharmaceuticals Research Center, Osaka, Japan

Purpose. This study was ­designed to inves­ti­gate the ­effect of a newly syn­the­sised sodi­um/hydro­gen ion ­exchange inhib­i­tor, SM-20550, on ischae­mia-reper­fu­sion ­induced inju­ry in a rat hind limb model.
Methods. In order to ­induce ischae­mia of the hind limbs, the abdom­i­nal aorta just dis­tal to the renal arter­ies and the bilat­er­al femo­ral arter­ies were ­clamped. Nineteen rats were divid­ed into three ­groups. In the sham group (n=5), the ves­sels were only dis­sect­ed and a vehi­cle solu­tion was admin­is­tered. In the con­trol group (n=7), the ves­sels were ­clamped for five hours, and a vehi­cle solu­tion was admin­is­tered 10 min­utes prior to clamp­ing and con­tin­ued for five hours after reper­fu­sion. In the SM group (n=7), clamp­ing was main­tained for five hours with a bolus injec­tion of SM-20550 and con­tin­u­ous infu­sion of the solu­tion for five hours after reper­fu­sion. Water con­tent of the left gas­troc­ne­mi­us mus­cle was cal­cu­lat­ed. The right gas­troc­ne­mi­us was fixed in 10% for­mal­in. A trans­verse thin sec­tion was ­stained with anti- myo­glo­bin anti­body. Stained cells of the right gas­troc­ne­mi­us were count­ed and the myo­glo­bin stain­ing index was cal­cu­lat­ed.
Results. Water con­tent was sig­nif­i­cant­ly (p<0.002) lower in the SM group than in the con­trol group. The myo­glo­bin stain­ing index was sig­nif­i­cant­ly (p<0.01) high­er in the SM group than in the con­trol group. There was no sig­nif­i­cant dif­fer­ence ­between the con­trol and the SM ­groups in crea­ti­nine phos­phok­i­nase (CPK) and lac­tate dehy­drog­e­nase (LDH).
Conclusions. Our ­results indi­cate that the sodi­um/hydro­gen ion ­exchange inhib­i­tor, SM-20550, ame­li­o­rates oede­ma for­ma­tion and ischae­mia-reper­fu­sion ­induced inju­ry of the skel­e­tal mus­cle.