ORIGINAL ARTICLES   

International Angiology 1998 March;17(1):34-7

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English

Factor G pathway reactive activity (GPRA) after surgery for abdominal aortic aneurysm

Sugita T., Watarida S., Katsuyama K., Nakajima Y., Yamamoto R., Mori A.

From the Second Department of Surgery, Shiga University of Medical Science, Otsu, Japan and * Department of Cardiovascular Surgery, Tenri Hospital, Tenri, Nara, Japan

Background. Following major sur­gery, detec­tion of endo­tox­ae­mia using the Toxicolor (Lim­u­lus) test has been report­ed. In addi­tion to endo­tox­ins, this test ­detects a reac­tant from human tis­sue, fac­tor G path­way reac­tive activ­ity (GPRA). We meas­ured endo­tox­in and GPRA in 10 ­patients dur­ing and after elec­tive sur­gery for abdom­i­nal aor­tic aneu­rysm. Additionally, we meas­ured phos­phok­i­nase (CPK) and GPRA lev­els in the mus­cle of 10 ­patients dur­ing abdom­i­nal aor­tic aneu­rysm sur­gery and 6 dur­ing open lap­a­rot­o­my for other caus­es.
Methods. Samples were taken from the arterial lines prior to sur­gery, ­before cross-clamp­ing of the aorta, imme­di­ate­ly after and 1, 3 6, and 18 hours after declamp­ing. Muscle spec­i­mens were taken from the rec­tus abdom­i­nus and homog­e­nized in many lev­els. Endotoxin con­cen­tra­tions were meas­ured with the Endotoxin-Specific test. GPRA con­cen­tra­tions were deter­mined by sub­tract­ing the val­ues from the Endotoxin-Specific test from those given by the Toxicolor test (Lim­u­lus assay with ach­rom­o­gen­ic sub­strate). CPK was also meas­ured.
Results. Endotoxin did not sig­nif­i­cant­ly ­increase dur­ing or after sur­gery, but GPRA was ele­vat­ed. GPRA in mus­cle cor­re­lat­ed sig­nif­i­cant­ly with CPK in mus­cle.
Conclusions. We con­clude that GPRA was ele­vat­ed after sur­gery, while endo­tox­in did not ­increase sig­nif­i­cant­ly. The GPRA prob­ably orig­i­nat­ed from human mus­cle tis­sue.