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Gazzetta Medica Italiana - Archivio per le Scienze Mediche 2019 March;178(3):163-6

DOI: 10.23736/S0393-3660.18.03746-4

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Cryptococcus liquefaciens meningitis in a patient with T cell non Hodgin lymphoma

Nida ÖZCAN 1, Tuba DAL 2 , Nezahat AKPOLAT 1, Rıza DURMAZ 2, Salim YAKUT 1, Hasan ZEYBEK 2, Kadri GÜL 1

1 Department of Microbiology, Dicle University Medical Faculty, Diyarbakır, Turkey; 2 Department of Microbiology, Ankara Yıldırım Beyazıt University Medical Faculty, Ankara, Turkey



Invasive fungal cryptococcosis including meningitidis is a growing threat to immunocompromised patients. Cryptococcus neoformans and Cryptococcus gattii are the main agents of human cryptococcosis. In recent years, there has been an increased incidence of opportunistic infections caused by other species, such as C. liquefaciens. In this study, we presented a meningitis case report caused by Cryptococcus liquefaciens in a 12 years old girl with T cell NHL (Non Hodgin lymphoma). A 12-year-old female patient with a diagnosis of T-NHL (T cell Non-Hodgkin’s lymphoma) under the chemotherapy was hospitalized in Hematology Clinic with the complaints of fever and occasional headache for three days. The yeast colonies were isolated from cerebrospinal fluid material (CSF). MALDI-TOF MS (Matriks assisted laser desorption ionization time of flight mass spectrometry, Bruker Daltonics, USA) and Nested PCR (polymerase chain reaction) were used to identify the yeast. Isolated yeast is identified as Cryptococcus liquefaciens by MALDI-TOF MS and Nested PCR. In conclusion, C. liquefaciens can be a causative agent of menigitidis in cases with NHL. Nested PCR was a good, time-saving and economical method for the accurate and early diagnosis of C. liquefaciens infections. Our presented case also showed that result of MALDI-TOF method was compatible with nested PCR method in identification of C. liquefaciens.


KEY WORDS: Cryptococcus - Polymerase chain reaction - Meningitis

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