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Gazzetta Medica Italiana Archivio per le Scienze Mediche 2018 March;177(3 Suppl 1):123-30

DOI: 10.23736/S0393-3660.18.03809-3

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Effects of emotional stress on plasma and saliva adrenalin and noradrenaline in students

Evgenij ZAKHAROV, Lada SMELYSHEVA , Natalya ARTENIAN, Irina KOTENKO

Kurgan State University, Kurgan, Russia


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BACKGROUND: In the modern world, the majority of actions performed by a human depend on his individual peculiarities: psychological, psychophysiological etc. One of the main reasons provoking nervous tension is the stress connected with exams. The aim of our study is to reveal the influence of the tone of the vegetative nervous system on the content of mediators in the sympathetic system using blood plasma and saliva and to establish a correlation between their secretion and excretion.
METHODS: The study involved 47 students of the Kurgan State University (40 female students and 7 male students) aged 18-21 during the semester and exam period (emotional stress model). At the beginning, all participants of the study were examined using Varikard 2.51 computer appliance. As a result of the analysis all participants were divided into groups depending on the tone of the vegetative nervous system (vagotony, normotony, sympathotony). The results of HRV analysis were interpreted using the spectral analysis. All participants of the experiment were divided into two groups (wave power below and above the average value). We took blood and saliva samples of all participants to detect hormone content in plasma and saliva. We estimated the concentration of adrenaline and noradrenaline in blood plasma and saliva using the method of enzyme-linked immunosorbent assay (ELISA) with DRG (Germany) tools.
RESULTS: After dividing the students according to their tone, it was revealed that the content of noradrenaline in blood under stress decreased in all groups, while the content of adrenaline decreased only in groups of normotonics and vagotonics. In saliva under stress, we registered the increased content of adrenaline in sympathotonics and the increased content of noradrenaline and saliva amount in vagotonics. After dividing the students according to their heart spectrum, it was revealed that under stress in all groups the content of catecholamines in blood decreased. In saliva under stress, we revealed the decreased content of noradrenaline and saliva amount in the group of students with a wave frequency below the average value and the increased content of noradrenaline and saliva amount in the group of students with a wave frequency above the average.
CONCLUSIONS: The assessment of catecholamines concentration in saliva and blood plasma as well as the data obtained as a result of the statistical and spectral analyzes allow us to suppose that the content of adrenaline and noradrenaline in saliva and blood depends on the initial tone of the vegetative system and HR spectrum.


KEY WORDS: Autonomic nervous system - Catecholamines - Adrenal glands - Epinephrine - Norepinephrine - Spectrum analysis

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