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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE

A Journal on Internal Medicine and Pharmacology


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Gazzetta Medica Italiana Archivio per le Scienze Mediche 2018 March;177(3):79-87

DOI: 10.23736/S0393-3660.17.03481-7

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Intense aerobic exercise modifies leucocytes number, lymphocyte subpopulation and cytokine levels in peripheral blood

Albená NUNES-SILVA 1 , Janaina MATOS MOREIRA 2, Leandro C. FREITAS LIMA 3, Elisa COUTO GOMES 4, Jose A. NOGUEIRA-MACHADO 5, Erica L. MARCIANO VIEIRA 2, Antonio L. TEIXEIRA 2

1 Sports Center, Federal University of Ouro Preto (CEDUFOP), Ouro Preto, Brazil; 2 Interdisciplinary Laboratory of Medical Research, Faculty of Medicine, Belo Horizonte, Brazil; 3 Institute of Biological Sciences of the Federal University of Minas Gerais, Belo Horizonte, Brazil; 4 Edinburgh Napier University (School of Life, Sport and Social Sciences), Scotland, UK; 5 Laboratory of Immunology, Institute of Ensino e Pesquisa da Santa Casa de Misericórdia, Belo Horizonte, Brazil


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BACKGROUND: The number of recreational runners has increased worldwide, and in Brazil, the number of racers who have completed running races has nearly tripled over the last decade. However, there is a limited amount of data demonstrating the relationship between running race stress and the immune system response. The aim of this study was to investigate the effects of a running race (10 km) on the physiological and immunological responses in healthy, well-trained male volunteers.
METHODS: Fourteen male participants (32.21±10.24 years old, 78.80±9.30 kg) participated in this study. Ratings of perceived exertion (RPE), visual analog scale (VAS), heart rate (HR) and blood samples were obtained before, immediately and 24 hours after the race. Lactate, glucose, creatine kinase (CK), leukocyte number and the subpopulation of T cells (CD4+ and CD8+) were analyzed.
RESULTS: Participants completed this race in 49.85±7.04 min. There was a significant increase in HR (67±9 to 159±21 bpm), RPE (6±0 to 15±2) and lactate (3.6 to 6.6 mmol/dL) post-race compared to pre-race. Glucose levels did not present any significant changes. The CK levels did not change immediately after the race but were higher (131.21±62.50 to 286.85±234.35 U/L) at the 24 h post-race time point. CRP was higher both immediately and 24 h after the race (4.5±2.3 to 8.36±2.28 and 8.37±2.29 mg/dL, respectively). The VAS values changed from 0 (before) to 5.64±2.20 (immediately after) to 2.21±2.86 (24 hours). The number of circulating leukocytes (5.83±0.89 to 9.15±1.77 103/µL), neutrophils (2.96±0.49 to 4.34±0.73 103/µL), lymphocytes (2.21±0.57 to 3.92±1.27 103/µL), monocytes (0.46±0.10 to 0.64±0.23 103/µL) and basophils (0.05±0.02 to 0.09±0.03 103/µL) increased significantly immediately after the race, returning to basal levels within 24 h. There was no difference in the number of circulating eosinophils. The absolute number of CD4+ (828.5±215.8 to 1063.2±235.3 cell/µL) and CD8+ (766.92±347.79 to 1470.30±782.90 cell/µL) also increased immediately after the race and returned to basal levels within 24 h. A significant reduction in the CD4+/CD8+ lymphocyte subpopulation ratio (1.21±0.45 to 0.85±0.33 cell/µL) was observed post-race and returned to basal levels at 24 h post-race. IL-6 (1.2±0.18 to 2.24±0.56 pg/mL) and IL-10 (1.8±0.16 to 2.5±0,93 pg/mL) also increased immediately after the running race; however, the IFN, IL-2, IL-4 and TNF levels did not change.
CONCLUSIONS: Taken together, these results suggested that intense aerobic exercise induces physiological changes and provokes significant, although transient, modulation of the immune system, specifically of the leukocyte sub-population.


KEY WORDS: Exercise - Immune system phenomena - Inflammation

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Publication History

Issue published online: February 22, 2018
Manuscript accepted: March 10, 2017
Manuscript received: January 27, 2017

Cite this article as

Nunes-Silva A, Matos Moreira J, Freitas Lima LC, Couto Gomes E, Nogueira-Machado JA, Marciano Vieira EL, et al. Intense aerobic exercise modifies leucocytes number, lymphocyte subpopulation and cytokine levels in peripheral blood. Gazz Med Ital - Arch Sci Med 2018;177:79-87. DOI: 10.23736/S0393-3660.17.03481-7

Corresponding author e-mail

albenanunes@hotmail.com