ORIGINAL ARTICLE   

Gazzetta Medica Italiana Archivio per le Scienze Mediche 2017 January-February;176(1-2):50-6

DOI: 10.23736/S0393-3660.16.03284-8

Copyright © 2015 EDIZIONI MINERVA MEDICA

language: English

A comparison of different methods to isolating primordial germ cells

Mohsen NAEEMIPOUR ✉

Department of Biochemistry and Nutrition, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran

BACKGROUND: Role of PGCs for producing transgenic chickens has significantly increased recently as a result of their exclusives capabilities. Primordial germ cells (PGCs) isolation in high numbers is the first step toward transgenic chicken production.
METHODS: In the present study we used the main methods to purify chicken PGCs including the isolating of PGCs with ammonium chloride-potassium (ACK) buffer, Ficoll and percoll density gradient centrifugation, and using the Magnetic cell sorting (MACS). We compared the methods based on the number of PGCs obtained.
RESULTS: Our results show that Ficoll density gradient centrifugation method is the preferential method.
CONCLUSIONS: There is a demand for a more suitable method for more efficient PGCs isolation.

KEY WORDS: Embryonic germ cells - Ficoll - Cell separation