ORIGINAL ARTICLES   

Gazzetta Medica Italiana Archivio per le Scienze Mediche 2012 December;171(6):795-805

Copyright © 2012 EDIZIONI MINERVA MEDICA

language: Italian

Evaluation of activity and toxicity of a 0.05% Electrolytic sodium hypochlorite solution in the tissue repair process

Santo G., D’atanasio N., Capezzone De Joannon A., Durando L., Consari I., Tongiani S., Milanese C.

ACRAF – Angelini Research Center, Santa Palomba, Pomezia, Roma

AIM: The aim of the present work was to evaluate the antimicrobial activity and the toxicity of a 0.05% electrolytic sodium hypochlorite solution in the tissue repair process.
METHODS: A re-epitheliazation model in mice has been used where the experimental wound was treated with 10 ml of saline solution or of a 0.05% electrolytic sodium hypochlorite solution twice a day for 12 days. In this period wound closure times and relative changes in cicatrization-dependent cell subpopulations (leukocytes and granulocytes/neutrophils) have been evaluated through morphometric and cytofluorimetric analysis. The cytotoxic and pro-inflammatory activities of a 0.05% electrolytic sodium hypochlorite solution were analysed in an in vitro model of cultured human skin where LDH release and TNF gene expression have been measured.
RESULTS: Treatment with a 0.05% electrolytic sodium hypochlorite solution does not affect wound closing times in comparison with controls treated with saline, neither in morphometric terms nor as far as cell subpopulation cytotoxicity is concerned. Such results have been confirmed in the in vitro human skin test where the amount of LDH released following treatment with a 0.05% electrolytic sodium hypochlorite solution is not different from that released in saline-treated controls and where no significant changes in TNF gene expression levels are observed.
CONCLUSION: The results of the study show that after repeated treatment, a 0.05% electrolytic sodium hypochlorite solution is devoid of cytotoxic and pro-inflammatory activity and does not interfere with the mechanisms responsible for wound re-epitheliazation and cicatrization, confirming both its efficacy and safety.