ORIGINAL ARTICLES   

Gazzetta Medica Italiana Archivio per le Scienze Mediche 2009 August;168(4):241-5

Copyright © 2009 EDIZIONI MINERVA MEDICA

language: English

Urine particles examination: comparison of automated flow citometer UF-1000i and manual quantitative microscopy

Manoni F., Ferrian M., Gusella G., Ercolin M., Tinello A., Fornasiero L., Valverde S., Gessoni G.

¹ Clinical Pathology Department, Ospedale Civile Monselice, Pordenone, Italy 2 Service of Laboratory Medicine, Ospedale Civile Chioggia, Venezia, Italy

Aim. Some automated instruments examining urine sediment have been introduced. We compared the performance of Sysmex UF-1000i with manual microscopy in urine particles examination.
Methods. 15 urine samples were selected. Urine sediments were examined by manual microscopy by using Fuchs-Rosenthal chamber and UF-1000i analyzer.
Results. The within-run CVs for urine samples ranged from 0.8% to 23.1% for the UF-1000i. The agreement between methods was good for red blood cells and white blood cells counts based on r values of 0.99, for epithelial cell the r value was 0.98 and 0.87 for casts.
Conclusion. This automated urinalysis system demonstrated good concordance with manual quantitative microscopy. Thus, when combined with urine chemistry analysis, this analyzer might provide a rapid and accurate screening tool in routine urine analysis.