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Gazzetta Medica Italiana Archivio per le Scienze Mediche 2004 April;163(2):49-58


language: English

Mineral and biochemical measurements during acute and rigorous bed rest to disclose biochemical change during acute bed rest in healthy subjects

Zorbas Y. G., Kakurin V. J., Charapakhin K. P., Kuznetsov N. A.

European Foundation of Environmental Sciences, Athens, Greece


Aim. The aim of ­this ­work was to meas­ure ­fluid absorption, plas­ma and uri­nary bio­chem­i­cal indi­ces dur­ing ­acute rig­or­ous bed ­rest (ABR). The objective of the study was also to dis­close if ABR ­or rig­or­ous bed ­rest (RBR) ­could ­affect sig­nif­i­cant­ly ­more ­fluid reten­tion and con­se­quent­ly bio­chem­i­cal var­i­ables in ­healthy sub­jects.
Meth­ods. Stud­ies ­were con­duct­ed dur­ing 3-day pre-bed ­rest (BR) and dur­ing 7-­days of ABR and RBR peri­ods. Thir­ty ­healthy ­male indi­vid­u­als, 25.2±6.4 ­years of age, ­were cho­sen as sub­jects. ­They ­were divid­ed equal­ly ­into 3 ­groups: unre­strict­ed ­active con­trol sub­jects (­UACS), ­acute bed rest­ed ­subjects (­ABRS) and rig­or­ous bed rest­ed sub­jects (­RBRS). ­ABRS con­fined abrupt­ly to RBR, and ­they did not ­have any ­prior knowl­edge of the ­exact ­date and ­time ­when ­they ­would be ­asked to be con­fined in bed. ­RBRS ­were sub­ject­ed to RBR on a pre­de­ter­mined ­date and ­time ­known to ­them ­right ­from the ­start of the ­study. ­UACS ­were not sub­ject­ed to any ­form of BR.
­Results. Uri­nary and plas­ma sodi­um (Na+), potas­sium (K+), cal­cium (Ca2+), mag­ne­sium (Mg2+) lev­els, ­whole ­blood hae­mog­lo­bin (Hb), hem­a­toc­rit (Hot), plas­ma ­renin activ­ity (PA), plas­ma aldos­te­rone (PA), uri­nary excre­tion, uri­nary crea­ti­nine and ­urea lev­els ­increased sig­nif­i­cant­ly (p<0.05), ­while ­fluid absorp­tion, uri­nary osmo­lar­ity and ­fluid con­sump­tion ­decreased sig­nif­i­cant­ly (p<0.05) in ­ABRS and ­RBRS ­when com­pared ­with ­their pre-bed ­rest val­ues and the val­ues in ­UACS. How­ev­er, ­fluid absorption, uri­nary and plas­ma elec­tro­lytes and bio­chem­i­cal var­i­ables ­changed sig­nif­i­cant­ly (p<0.05) ­more and sig­nif­i­cant­ly (p<0.05) fast­er in ­ABRS ­than ­RBRS. Con­verse­ly, uri­nary and plas­ma Na+, K+, Ca2+, and Mg2+ lev­els, ­whole ­blood Hb, Hct, PRA, PA, uri­nary osmo­lar­ity, crea­ti­nine, ­urea lev­els, ­fluid absorp­tion, ­fluid con­sump­tion and ­fluid elim­i­na­tion ­leves did not ­change in ­UACS com­pared ­with ­their pre-bed ­rest val­ues.
Con­clu­sion. The sig­nif­i­cant­ly great­er bio­chem­i­cal chang­es in ­ABRS ­than ­RBRS may dem­on­strate ­that plas­ma and uri­nary bio­chem­is­try is affect­ed sig­nif­i­cant­ly ­more in ­ABRS ­than ­RBRS. It was con­clud­ed, the ­more abrupt­ly mus­cu­lar activ­ity is restrict­ed the great­er elec­tro­lyte and bio­chem­i­cal chang­es in ­healthy sub­jects and prob­ably in indi­vid­u­als ­whose mus­cu­lar activ­ity is abrupt­ly ter­mi­nat­ed.

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