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ORIGINAL ARTICLE   Free accessfree

Esperienze Dermatologiche 2019 March;21(1):22-6

DOI: 10.23736/S1128-9155.19.00477-1


language: English

Optimization of the lipofilling procedure with hybrid cooperative complexes of high and low molecular weight hyaluronic acid: preliminary experiments

Antonello TATEO 1, 2, 3 , Giuseppe SOFO 1

1 TBClinic Milano Aesthetic Surgery and Medicine, Milan, Italy; 2 Unit of Plastic Surgery, Istituto Auxologico Italiano IRCCS, Centro Diagnostico San Carlo-Capitanio, Milan, Italy; 3 Breast Unit, Clinica San Carlo, Paderno Dugnano, Milan, Italy


BACKGROUND: Efforts have been made in recent years to standardize the lipofilling procedure in order to improve adipose tissue engraftment at the recipient site in an attempt to achieve a better volumizing result months after surgery. This procedure is, however, still burdened by the resorption of variable amounts of the graft. Recently, a new hyaluronic acid made up of hybrid cooperative complexes (HCCs) has demonstrated an ability to increase the differentiation and proliferation of adipocyte-derived stem cells (ASCs). Based on recent evidence regarding fat grafts and hyaluronic acid, this preliminary experiment was carried out to verify whether the two implant materials may be complementary. The aim of this pilot study was therefore to test a hypothetical complementary approach between lipofilling and hyaluronic acid hybrid cooperative complexes to suggest a possible solution to the problem of graft resorption in the adipose transplantation procedure. This preliminary experiment has confirmed the effectiveness of HCCs on the proliferation of ASCs and the preservation of adipocyte vitality.
METHODS: Thirteen patients were enrolled in this study and four laboratory test were performed.
RESULTS: The sample treated with the closed-circuit washing system was the most concentrated in terms of percentage of adipose tissue (60%) compared to the control (55%). The sample purified with the same system and with the addition of 5% HCCs, although less concentrated (58%) than the sample without additions, was still more concentrated than the control (55%). The sample with 10% HCCs added presented adipocyte concentrations comparable to the control.
CONCLUSIONS: Based on the results obtained, the efficacy of HCCs on the proliferation of ASCs and on the preservation of the vitality of adipocytes is confirmed.

KEY WORDS: Hyaluronic acid; Adipocytes; Adipose tissue; Transplantation

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