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Esperienze Dermatologiche 2017 June-December;19(2-4):53-9

DOI: 10.23736/S1128-9155.17.00450-2


language: English

The multicomponent powder (Vitachelox™) rich in polyphenols protects skin cells against the toxic effects of polluting agents

Luca GIACOMELLI 1 , Stefano TOGNI 2, Martino MENEGHIN 2, Roberto EGGENHOFFNER 1, Giada MARAMALDI 2

1 Department of Surgical Science and Integrated Diagnostics, University of Genoa, Genoa, Italy; 2 Indena S.p.A., Milan, Italy


BACKGROUND: Although healthy skin is well equipped to counteract environmental exposure, air pollution could affect its protective potential. Based on their antioxidant, anti-inflammatory and antimicrobial properties, polyphenols could represent a promising tool in preventing skin damage and the development of various skin disorders. In this light, a multicomponent powder (Vitachelox™) including three naturally-occurring extracts rich in polyphenols has been developed and tested in fibroblast cell culture.
METHODS: In this in vitro study, we evaluated the skin protective activity of Vitis vinifera (grape) seed extract, Camellia sinensis leaf (green tea) extract, Quercus robur (oak) wood/bark extract, both as single agents and as multi-component powder (Vitachelox™) in fibroblast cell culture exposed to lead (Pb), iron (Fe) and chromium (Cr). In particular, we performed the following tests: MTT test, Lowry assay, lipid peroxidation assay, protein carbonyl content assay and DNA damage quantification assay.
RESULTS: In fibroblast cell cultures where environmental damage was experimentally induced, treatment with the naturally-occurring extracts both as single agents and as multicomponent powder counteracted the toxic effect of heavy metals, improving cell viability as well as total protein content. Noteworthy, Vitachelox™ showed a synergistic effect, increasing cell viability over 77% (in comparison with the positive control) while the protective effect exerted by each single agent was 62.68% for grape seed extract, 44.92% for green tea extract and 38.44% for oak wood extract. In addition, the multi-component powder protected fibroblasts against the heavy metal-induced oxidative stress, by restoring the total amount of proteins and preserving the cellular components (lipids, proteins and DNA).
CONCLUSIONS: These results indicated that the multicomponent powder (Vitachelox™) as well as its naturally-occurring ingredients (grape seed extract, green tea extract, oak wood/bark extract) protected fibroblast cell culture against the toxic effect of heavy metals, suggesting that it could play an effective role in counteracting skin damages induced by air pollution.

KEY WORDS: Air pollution - Metals, heavy - Oxidative stress - Green tea extract polyphenone E - Grape seed extract

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