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Esperienze Dermatologiche 2017 March;19(1):31-7

DOI: 10.23736/S1128-9155.17.00445-9


language: English

Evaluation of in-vitro anti-staphylococcal activity of fusidic acid and gentamicin

Marco TOSCANO 1 , Clementina COCUZZA 2, Lorenzo DRAGO 1, Roberto MATTINA 3, Paola A. PILLONI 4, Enrico CALARESU 2, Valentina COZZA 5, Adone BARONI 6

1 Department of Biomedical Health Sciences, University of Milan, Milan, Italy; 2 Department of Medicine and Surgery, University of Milan-Bicocca, Milan, Italy; 3 Department of Biomedical, Surgical and Dental Sciences, University of Milan, Milan, Italy; 4 Department of Experimental Medicine, Second University of Naples, Naples, Italy; 5 Department of Prevention, Tumor Registry, Local Health Authority of Naples, Naples, Italy; 6 Department of Dermatology and Venereology, Second University of Naples, Naples, Italy


BACKGROUND: Staphylococcus aureus is considered one of the main causes of skin and soft tissue infections. Such infections are generally treated with topical antibiotics as these can reach high concentrations at the infection sites. However, the significant increase of antibiotic resistance observed in recent years poses a serious problem in the choice of the most appropriate antimicrobial agent to be used. The purpose of this work was to evaluate the in-vitro antibacterial activity of fusidic acid and gentamicin against several clinical isolates of Staphylococcus spp. involved in skin and soft tissue infections.
METHODS: The following microorganisms were tested in this study: 30 strains of methicillin-resistant Staphylococcus aureus (MRSA), 30 strains of methicillin-susceptible Staphylococcus aureus (MSSA), 20 strains of methicillin-resistant Staphylococcus epidermidis (MRSE) and 20 strains of methicillin-susceptible Staphylococcus epidermidis (MSSE). All examined microorganisms were isolated from hospitalised patients with skin infections or soft tissue infections. The antibacterial activity of fusidic acid and gentamicin was evaluated by means of the agar diffusion method (Kirby-Bauer) against each of the Staphylococcus spp. clinical isolates.
RESULTS: Fusidic acid was effective against all tested strains of MRSA and MSSA, while gentamicin was ineffective against two MRSA strains and 2 MSSA strains. In addition, no strain of MRSE and MSSE was found to be resistant to fusidic acid; on the other hand, 3 MRSE strains and 2 MSSE strains were resistant to gentamicin.
CONCLUSIONS: Fusidic acid can be considered a first-line antibiotic for the treatment of skin and soft tissue infections as indicated by the excellent antibacterial activity demonstrated in this study combined with its high level of skin penetration.

KEY WORDS: Staphylococcus - Staphylococcal skin infections - Antibacterial agents

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