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Giornale Italiano di Dermatologia e Venereologia 2018 Oct 29

DOI: 10.23736/S0392-0488.18.06161-8

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Lack of detection of cutavirus DNA using PCR real time in Cutaneous T-cell Lymphomas (CTCL)

Massimiliano BERGALLO 1 , Valentina DAPRÀ 1, Paolo FAVA 2, Renata PONTI 2, Cristina CALVI 1, Maria Teresa FIERRO 2, Pietro QUAGLINO 2, Ilaria GALLIANO 1, Paola MONTANARI 1

1 Infectious Diseases Unit, Department of Pediatrics, Regina Margherita Children's Hospital, University of Turin, Turin, Italy; 2 Dermatology Section, Department of Medical Sciences, University of Turin, Turin, Italy


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BACKGROUND: A novel human protoparvovirus named cutavirus has been discovered. We investigated the presence of cutavirus in a sample of Cutaneous T-cell Lymphomas by using PCR real time taqman.
METHODS: In total, 55 CTCL samples were analyzed using a Taqman Real time PCR on a 7500 ABI instrument. All of these shown internal control amplification.
RESULTS: The presence of cutavirus DNA corresponding was examined. CuV DNA sequences were not detected in any skin specimen.
CONCLUSIONS: The role of cutaviruses in cutaneous cancers remains to be investigated.


KEY WORDS: Human protoparvovirus - Cutaneous T-cell lymphomas - Mycosis fungoides - Sézary syndrome

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