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GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA

A Journal on Dermatology and Sexually Transmitted Diseases


Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
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Giornale Italiano di Dermatologia e Venereologia 2017 Nov 30

DOI: 10.23736/S0392-0488.17.05726-1

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Milk thistle and olive extract: old substances with a new mission against sun-induced skin damage

Roberta DI CAPRIO 1 , Giuseppe MONFRECOLA 1, Franco GASPARRI 2, Raffaella MICILLO 1, Anna BALATO 3, Serena LEMBO 4

1 Department of Clinical Medicine and Surgery, Section of Dermatology, University of Naples Federico II, Naples, Italy; 2 Department of Pharmacy (DIFARMA), University of Salerno, Salerno, Italy; 3 Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy; 4 Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Salerno, Italy


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BACKGROUND: Natural antioxidants represent an effective option in the prevention and/or improvement of ultraviolet radiations (UVR)-induced/aggravated skin conditions. UVR cause DNA damage in keratinocytes, directly, in the form of cyclobutane pyrimidine dimers (CPDs), or indirectly, through oxidative stress production. Failure of the repair system can result in genetic mutations primarily responsible for the initiation of NMSCs. The aim of our study was to evaluate the in vitro protective effect of milk thistle and olive purified extracts on cultured keratinocytes after solar simulator irradiations (SSR).
METHODS: Immortalized keratinocytes were pre-incubated with different concentrations of milk thistle and olive purified extracts, and irradiated with increasing doses of SSR. Thereafter, CPDs and p53 expression were evaluated to assess DNA damage, whereas cellular antioxidants consumption and lipid membranes peroxidation were measured to analyse oxidative stress.
RESULTS: The study substances were well tolerated by cells and displayed good cytoprotective and anti-oxidant activities, being milk thistle dry extract more effective in limiting the direct DNA damage, and olive extract particularly able to reduce lipid membrane peroxidation and to increase cellular antioxidants.
CONCLUSIONS: Both study substances can be defined as safe compounds, showing differential cytoprotective and anti-oxidant activities and might represent interesting options for NMSCs chemoprevention.


KEY WORDS: Chemoprevention - Milk thistle dry extract - Purified olive extract - SSR

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Publication History

Article first published online: November 30, 2017
Manuscript accepted: October 5, 2017
Manuscript received: July 6, 2017

Cite this article as

Di Caprio R, Monfrecola G, Gasparri F, Micillo R, Balato A, Lembo S. Milk thistle and olive extract: old substances with a new mission against sun-induced skin damage. G Ital Dermatol Venereol 2017 Nov 30. DOI: 10.23736/S0392-0488.17.05726-1

Corresponding author e-mail

roberta.dicaprio@libero.it