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GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA

A Journal on Dermatology and Sexually Transmitted Diseases


Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
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Giornale Italiano di Dermatologia e Venereologia 2017 Sep 20

DOI: 10.23736/S0392-0488.17.05773-X

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

MiR-564 promotes hypertrophic scar via up-regulating TGF-β1

Long XIAO 1, Tianyi TANG 1, Yu HUANG 2, Jianxing GUO 1

1 Department of Orthopaedics, Zhangjiagang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, China; 2 Department of Obstetrics and Gynecology, Zhangjiagang First People's Hospital, The Zhangjiagang Affiliated Hospital of Soochow University, Zhangjiagang, China


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BACKGROUND: To evaluate the role of microRNA564 (miR-564) in the development of hypertrophic scar and investigate the possible mechanism of this process.
METHODS: The hypertrophic scar (HS) tissues and adjacent normal skin (NS) tissues were selected from 10 patients. The fibrosis-related proteins were detected via hematoxylin eosin (HE) staining, Masson staining and immunohistochemical detection. The relative expression difference of miR-564 in NS tissues and HS tissues was detected via reverse transcription-polymerase chain reaction (RT-PCR). The protein expression difference of transforming growth factor-β1 (TGF-β1) in NS tissues and HS tissues was detected via Western blotting. Moreover, the hypertrophic scar fibroblast (HSF) cells were isolated from HS tissues and divided into four groups according to different treatment methods: blank control group, scramble-transfected negative control group, miR-564 inhibitor-transfected miR-564 inhibitor group, and miR-564 plasmid-transfected miR-564 mimic group. The expressions of TGF-β1 in blank control group, scramble group, miR-21 mimic group and miR-21 inhibitor group were detected via RT-PCR and Western blotting.
RESULTS: MiR-564 was highly expressed in HSF cells. Compared with that of blank control group, the expression of TGF-β1 was down-regulated through inhibiting the miR-564 expression, thus inhibiting the activation and proliferation of HSF cells. However, the overexpression of miR-564 achieved the opposite results.
CONCLUSIONS: Up-regulated mir-564 promoted the development of hypertrophic scar via enhancing the expression of TGF-β1. MiR-564 may be a potential novel molecular target for the treatment of hypertrophic scarring.


KEY WORDS: MicroRNA-564 - TGF-β1 - Human hypertrophic scar fibroblasts - Cell proliferation

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Publication History

Article first published online: September 20, 2017
Manuscript accepted: September 5, 2017
Manuscript received: August 31, 2017

Cite this article as

Xiao L, Tang T, Huang Y, Guo J. MiR-564 promotes hypertrophic scar via up-regulating TGF-β1. G Ital Dermatol Venereol 2017 Sep 20. DOI: 10.23736/S0392-0488.17.05773-X

Corresponding author e-mail

terry1982126@sina.com