Home > Journals > Giornale Italiano di Dermatologia e Venereologia > Past Issues > Giornale Italiano di Dermatologia e Venereologia 2020 June;155(3) > Giornale Italiano di Dermatologia e Venereologia 2020 June;155(3):286-93

CURRENT ISSUE
 

JOURNAL TOOLS

eTOC
To subscribe
Submit an article
Recommend to your librarian
 

ARTICLE TOOLS

Publication history
Reprints
Permissions
Cite this article as

 

ORIGINAL ARTICLE   

Giornale Italiano di Dermatologia e Venereologia 2020 June;155(3):286-93

DOI: 10.23736/S0392-0488.17.05726-1

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Milk thistle and olive extract: old substances with a new mission against sun-induced skin damage

Roberta DI CAPRIO 1 , Giuseppe MONFRECOLA 1, Franco GASPARRI 2, Raffaella MICILLO 1, Anna BALATO 3, Serena LEMBO 4

1 Section of Dermatology, Department of Clinical Medicine and Surgery, Federico II University of Naples, Naples, Italy; 2 Department of Pharmacy (DIFARMA), University of Salerno, Salerno, Italy; 3 Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy; 4 Department of Medicine, Surgery and Dentistry, Scuola Medica Salernitana, University of Salerno, Salerno, Italy



BACKGROUND: Natural antioxidants represent an effective option in the prevention and/or improvement of ultraviolet radiations (UVR)-induced/aggravated skin conditions. UVR cause DNA damage in keratinocytes, directly, in the form of cyclobutane pyrimidine dimers (CPDs), or indirectly, through oxidative stress production. Failure of the repair system can result in genetic mutations primarily responsible for the initiation of NMSCs. The aim of our study was to evaluate the in vitro protective effect of milk thistle and olive purified extracts on cultured keratinocytes after solar simulator irradiations (SSR).
METHODS: Immortalized keratinocytes were pre-incubated with different concentrations of milk thistle and olive purified extracts, and irradiated with increasing doses of SSR. Thereafter, CPDs and p53 expression were evaluated to assess DNA damage, whereas cellular antioxidants consumption and lipid membranes peroxidation were measured to analyze oxidative stress.
RESULTS: The study substances were well tolerated by cells and displayed good cytoprotective and antioxidant activities, being milk thistle dry extract more effective in limiting the direct DNA damage, and olive extract particularly able to reduce lipid membrane peroxidation and to increase cellular antioxidants.
CONCLUSIONS: Both study substances can be defined as safe compounds, showing differential cytoprotective and antioxidant activities and might represent interesting options for NMSCs chemoprevention.


KEY WORDS: Chemoprevention; Milk thistle; Olive extract

top of page