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Giornale Italiano di Dermatologia e Venereologia 1998 October;133(5):325-7

Copyright © 1998 EDIZIONI MINERVA MEDICA

language: Italian

Modulation of leukotriene B4 metabolism from human polymorphonuclear granulocytes by naftifine

Campanile G. 1, Ludovici M. 2, Bianchi B. 1, Difonzo E. M. 1

1 Università degli Studi - Firenze, Istituto di Clinica Dermosifilopatica; 2 Dipartimento di Farmacologia Preclinica e Clinica


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Background. The accumulation of neutrophils in the infected skin is an early event during dermatomycosis. For this reason, the potential antiphlogistic activity of an antifungal drug, especially topical, supports its therapeutical effect very much, in particular in case of very phlogistic dermatophytoses. The antinflammatory activity of naftifine is studied, investigating its influence on the leukotriene B4 metabolism of human neutrophil granulocytes (PMN).
Methods. The cells were stimulated with the Ca-ionophore A23187 over 15 minutes in the presence of the drug. Subsequently the supernatants were analyzed for their leukotriene B4 content by reversed-phase HPLC.
Results. The results showed that naftifine inhibited the release of leukotriene B4 in PMN already at concentrations of 6.4 µ/ml. At concentrations of 640 µ/ml naftifine totally blocked the release of leukotrien B4 in PMN.
Conclusions. Further investigations should clarify what enzymes are important for the observed antinflammatory activity. The interaction with cytochrome-P450 by azoles cannot be accepted for naftifine which does not act at this level.

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