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Chirurgia 2021 August;34(4):141-53

DOI: 10.23736/S0394-9508.20.05129-3


language: Italian

Quantification of platelets and leukocytes in solid blood platelet concentrates for surgical use: study of an experimental technique

Alessandro CRISCI 1, 2 , Tomoyuki KAWASE 3, Raffaele D’ADAMO 2, Michela CRISCI 4

1 Scuola Medica Salernitana, Department of Medicine, Surgery and Dentistry, University of Salerno, Fisciano, Salerno, Italy; 2 Unit of Skin Surgery, Skin Transplants and Difficult Wounds, Villa Fiorita Nursing Home, Aversa, Caserta, Italy; 3 Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan; 4 Department of Surgery, Faculty of Medicine and Surgery, Vasile Goldis Western University of Arad, Arad, Romania

BACKGROUND: Neutrophil granulocytes and platelets are key in wound healing and, in combination with secreted growth factors/cytokines, they facilitate tissue regeneration and the formation of new blood vessels. Some studies showed a method for direct estimation of platelet counts in the PRF by using a recombinant t-PA through a digestion method.
METHODS: Authors have elaborated the data obtained in the figures and tables related to the work published by the Niigata University (Niigata, Japan) group and have evaluated the percentage deviation between “counts with t-PA” and PLT and WBC values with “counts for subtraction” and with “counts for blood count” with the use of a “statistical method.”
RESULTS: By reducing the value of leukocytes obtained by the subtraction method by 34.35%, the value reported with the t-PA method is obtained; reducing the value of the platelets in the PRF membrane obtained from the same method by 19.69%, the value reported with the t-PA method is obtained; reducing the value of leukocytes obtained from the blood count by 34.12%, the value reported with the t-PA method is obtained; and by reducing the value of the platelets obtained with the same method by 15.12%, the value reported with the method is obtained from digestion with t-PA.
CONCLUSIONS: With this experiment the authors made it possible to validate a simple and inexpensive system for calculating the precise number of platelets and leukocytes present in second-generation platelet concentrates, making it easy to evaluate individual PRF matrices in a timely manner in a clinical setting.

KEY WORDS: Platelet-rich fibrin; Blood platelets; Tissue-plasminogen activator

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