ORIGINAL ARTICLES   

Chirurgia 2015 October;28(5):163-7

Copyright © 2015 EDIZIONI MINERVA MEDICA

language: English

Inhibitory effect of celecoxib on intimal hyperplasia of venous autografts in a rat autogenous vein graft model

Wang M. ^{1, 2}, Li C. ¹, Chi C. ¹, Liu H. ¹

¹ Department of Cardiac Surgery, the First Affiliated Hospital, Harbin Medical University, Harbin, China; ² Department of Cardiothoracic Surgery, Guangdong No.2 Provincial People’s Hospital, Guangzhou, China

AIM: The aim of this paper was to establish a more easy-to-operate model that simulates the saphenous vein graft in coronary artery bypass graft surgery, and to explore the relationship between celecoxib and the proliferation of smooth muscle cells (VSMCs) in the vein graft.
METHODS: After intraperitoneal anesthesia with 10% chloral hydrate saline solution (40 mg/kg), a rat was operated to create two side to side anastomoses between the autologous inferior vena cava and aorta under microscope, and the vena cava was blocked at both ends of the anastomoses corresponding to the sites below the starting point of the renal vein and above the common iliac vein. A study model was thereby completed. Oral celecoxib (50 mg/kg·d) was given by forced gavage one day before surgery to two weeks after surgery, and a celecoxib group and a saline control group were established. Changes of the PCNA expression in VSMCs in the autologous vein graft were observed using pathological andPCNA immunohistochemical staining.
RESULTS: Vein grafts were successfully established in 36 rats without intraoperative or perioperative mortality. The overall graft patency was 82%. For patent grafts, morphological observation found statistical differences in intimal thickening between the two groups on days 1, 5, and 7 after surgery. Intimal thickening was evident and PCNA positive cells were significantly increased in the control group. In contrast, neointimal hyperplasia was significantly inhibited in the celecoxib group, and PCNA index was significantly lower than the saline control group (P<0.05).
CONCLUSION: This animal model can simulate the conditions of saphenous vein grafts in coronary artery bypass grafting, and is simple and easy to operate. Celecoxib inhibits PCNA expression in vein graft intimal hyperplasia.