ORIGINAL ARTICLES  CARDIAC PAPERS 

The Journal of Cardiovascular Surgery 2000 August;41(4):541-5

Copyright © 2009 EDIZIONI MINERVA MEDICA

language: English

Myocardial fixation of anticardiac troponin I antibody and cardiac troponin I release

Alwan K., Chocron S., Mourand J. L., Toubin G. ¹, Gauchez P. ², Kantelip B. ², Kantelip J. P. ¹, Etievent J. P.

From the Department of Thoracic and Cardiovascular Surgery 1 Department of Pharmacology 2 Department of Pathology Saint-Jacques Hospital, Besançon, France

Background. The three­fold aim of ­this experi­men­tal ­study was to ­test the cor­re­la­tion of car­diac tro­po­nin I ­released to myo­car­dial infarc­tion ­size and myo­car­dial fix­a­tion of anti­car­diac tro­po­nin I anti­body and to deter­mine how ­long ­after myo­car­dial infarc­tion the meas­ure of car­diac tro­po­nin I con­cen­tra­tion can eval­u­ate myo­car­dial infarc­tion ­size.
Methods. Forty rab­bits ­were ­assigned ­either to a con­trol ­group or to an experi­men­tal pre­con­di­tioned ­group. Infarction was ­obtained by tight­en­ing a ­snare ­around the ­left ante­ri­or descend­ing ­artery. Serial ­venous ­blood sam­ples ­were ­drawn for meas­ure­ment of car­diac tro­po­nin I. The rab­bits ­were sac­ri­ficed at 72 ­hours and a his­to­log­i­cal ­study was per­formed to deter­mine the ­infarct ­size and the ­size of the ­area ­void of fix­a­tion of anti­car­diac tro­po­nin I anti­body.
Results. There was a lin­e­ar cor­re­la­tion ­between the ­total ­amount of CTn I ­released and ­both ­infarct ­size (r=0.45, p<0.02) and the ­size of the ­area ­void of ­anti-car­diac tro­po­nin I anti­body (r=0.47, p<0.02). These two siz­es ­were strong­ly cor­re­lat­ed (r=0.95, p<0.02). The ­hour 9 CTn I sam­ple was the ­best cor­re­lat­ed ­with ­both the ­infarct ­size (r=0.47, p<0.02) and the ­size of ­area ­void of anti­car­diac tro­po­nin I anti­body (r=0.45, p<0.02).
Conclusions. Our ­study ­shows ­that: 1) car­diac tro­po­nin I ­release is cor­re­lat­ed to ­both myo­car­dial infarc­tion ­size and the ­size of ­area ­void of fix­a­tion of anti­car­diac tro­po­nin I anti­body, 2) the ­area ­void of anti­car­diac tro­po­nin I anti­body fix­a­tion ­includes the ­whole ischem­ic ­area, and 3) eval­u­a­tion of myo­car­dial infarc­tion ­size can be ­obtained by CTn I con­cen­tra­tion as ear­ly as the ­ninth ­hour.