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Acta Vulnologica 2016 December;14(4):196-201


language: English, Italian

Effects of a spring water on human skin fibroblast in vitro cultures: preliminary results

Giovanni NICOLETTI 1, 2, 3, Marco SALER 1, Tommaso PELLEGATTA 1, 2, Alberto MALOVINI 4, Angela FAGA 1, 2, 3, Alessandro SCALISE 5, Federica RIVA 6

1 Plastic Surgery Unit, Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy; 2 Plastic and Reconstructive Surgery Unit, Salvatore Maugeri Foundation and Institute for Research and Care, Pavia, Italy; 3 Research Center for Advanced Technologies in Regenerative Medicine and Inductive Surgery, University of Pavia, Pavia, Italy; 4 Laboratory of Informatics and Systems Engineering for Clinical Research, Salvatore Maugeri Foundation and Institute for Research and Care, Pavia, Italy; 5 Clinic of Plastic and Reconstructive Surgery, Polytechnic University of Marche, Ancona, Italy; 6 Histology and Embryology Unit, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, Italy


BACKGROUND: A previous in vivo animal wound model proved that a bicarbonate-calcium-magnesium spring water (Comano, Trento, Italy) improved skin regeneration. This in vitro trial evaluated the effects of the same spring water on human skin fibroblasts.
METHODS: The fibroblasts were cultured in Dulbecco’s Modified Eagle Medium (DMEM) enriched with fetal bovine serum, penicillin, streptomycin, gentamicin and L-glutamine and in DMEM diluted with spring water at different concentrations.
RESULTS: Our results suggest the active role of different partial replacements of DMEM with the Comano spring water in regulating the vitality of the human fibroblast cultures. The 20% replacement of DMEM with spring water demonstrated a relevant increase in the cell vitality vs. the cultures with DMEM alone. This favorable effect seems to start at 48 hours, peaks at 72 hours and persists up to 7 days.
CONCLUSIONS: These effects match the results of the previous in vivo animal study.

KEY WORDS: Cell culture techniques - Fibroblasts - Microbiota - Regeneration - Hot springs

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