Home > Journals > Acta Vulnologica > Past Issues > Acta Vulnologica 2006 March;4(1) > Acta Vulnologica 2006 March;4(1):27-47






Acta Vulnologica 2006 March;4(1):27-47


language: Italian

Cultured skin substitutes in the treatment of cutaneous skin wounds

Marazzi M. 1, Chiaratti A. 1, Falcone L. 1, Scalise A. 2, Crovato F. 1, Calò D. 1, Ordanini M. N. 1, Stefani A. 1, Pierangeli M. 2, Astolfi M. 2, Rapisarda V. 3, Bertani A. 2

1 Centro di Riferimento Regionale per la Coltura di Epidermide Umana in Vitro e Centro per la Crioconservazione dei tessuti S.S. Terapia Tissutale 2 Clinica di Chirurgia Plastica e Ricostruttiva Università Politecnica delle Marche, Ancona 3 S.C. Chirurgia Plastica e Centro Grandi Ustionati Azienda Ospedaliera Ospedale Niguarda Ca’ Granda, Milano


Skin tissue engineering relies on multidisciplinary research to develop innovative therapies that can overcome the drawbacks to skin transplants and grafts and to create functional and compatible biological substitutes for the repair of damaged tissue or to replace tissue loss. Tissue engineering techniques offer the possibility to repair extensive solutions of continuity of the skin, where loss of skin integrity exposes the body to the risk of homeostatic imbalance, external injury and attack by microorganisms. Despite numerous attempts to cultivate human epidermal basal cells, the method described by Rheinwald and Green remains the most reliable and widely used technique for cultivating large quantities of multilayered epidermal sheets for autologous dermal and epidermal grafts. With this study we evaluated various keratinocyte culturing methods. Here we describe the different types of scaffolds for preparing dermal substitutes, focusing on a method based on the Rheinwald-Green keratinocyte culturing technique and on the use of a semisynthetic polysaccharide consisting of a benzyl ester of hyaluronic acid (HYAFF®) obtained by 100% esterification and employed as a scaffold for culturing epithelial cells. We also present the clinical results obtained with the application of these skin substitutes at the outpatient services for severe injuries at the Niguarda Ca’Grande Hospital, Milan, in collaboration with the center for severe injuries of the Ospedali Riuniti, Ancona. The results suggest that epidermal basal cells cultivated in vitro play an increasingly important role in the treatment of cutaneous wounds.

top of page