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Italian Journal of Dermatology and Venereology 2021 Jul 20

DOI: 10.23736/S2784-8671.21.06953-X


language: English

CD27 mRNA expression in mycosis fungoides

Ilaria GALLIANO 1, Valentina DAPRÀ 1, Renata PONTI 2, Carla ALLIAUDI 1, Maria T. FIERRO 2, Pietro QUAGLINO 2, Massimiliano BERGALLO 1

1 Infectious Diseases Unit, Department of Pediatrics, Regina Margherita Children's Hospital, University of Turin, Turin, Italy; 2 Dermatology Section, Department of Medical Sciences, University of Turin, Turin, Italy


BACKGROUND: The etiopathogenesis of MF remains obscure. CD27 is a member of the tumor necrosis factor receptor superfamily (TNFRS) that regulates lymphocyte function4. Expression of CD27 protein and mRNA has been reported in B-cell lymphomas5 and adult T-cell leukemia/lymphoma6. In this study, we examined the expression of CD27 in the skin of MF patients by real time PCR. The amount of CD27 was measured in MF patients and healthy controls.
METHODS: A total of 98 skin biopsies were analyzed: 12 obtained from healthy donors and 86 obtained Cryostatic sections OCT-embedded affected by MF. Relative quantification of mRNA CD27 expression was achieved by means of TaqMan amplification and normalization to glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
RESULTS: Housekeeping gene was detectable in all Skin samples and there isn’t difference between healthy control and MF p value 0.1564. CD27 mRNA sequences were found in 3 of 12 (25%) of skin obtained from healthy donors and in 59 of 86 (68%) of skin obtained from Cryostatic sections OCT-embedded affected by MF. The chi-square statistic with Yates correction is 6.8413 and the p-value is 0.0089. When we compared the CD27 expression in MF and controls the RQ analysis show a value of 9.12±14.13. A RQ of 9.12 means that this gene is 9.12 times more expressed in MF skin samples then in the healthy skin samples. No difference were observed in the MF clustered by stages.
CONCLUSIONS: Our findings indicates that CD27 can be used as diagnostic/prognostic markers, and whether anti-CD27 antibodies can be used in therapy.

KEY WORDS: Cutaneous T-cell lymphomas; Mycosis fungoides; TNFRS; CD27

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