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ORIGINAL ARTICLE   

Giornale Italiano di Dermatologia e Venereologia 2020 December;155(6):772-4

DOI: 10.23736/S0392-0488.18.06161-8

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Lack of detection of Cutavirus DNA using PCR real time in cutaneous T-cell lymphomas

Massimiliano BERGALLO 1 , Valentina DAPRÀ 1, Paolo FAVA 2, Renata PONTI 2, Cristina CALVI 1, Maria T. FIERRO 2, Pietro QUAGLINO 2, Ilaria GALLIANO 1, Paola MONTANARI 1

1 Unit of Infectious Diseases, Department of Pediatrics, Regina Margherita Children’s Hospital, University of Turin, Turin, Italy; 2 Dermatology Section, Department of Medical Sciences, University of Turin, Turin, Italy



BACKGROUND: A novel human protoparvovirus named Cutavirus has been discovered. We investigated the presence of Cutavirus in a sample of Cutaneous T-cell lymphomas by using PCR real time TaqMan® (Thermo Fisher Scientific, Waltham, MA, USA).
METHODS: In total, 55 CTCL samples were analyzed using a TaqMan® Real time PCR on a 7500 ABI instrument. All of these shown internal control amplification.
RESULTS: The presence of Cutavirus DNA corresponding was examined. CuV DNA sequences were not detected in any skin specimen.
CONCLUSIONS: The role of Cutaviruses in cutaneous cancers remains to be investigated.


KEY WORDS: Parvovirus; Lymphoma, T-cell, cutaneous; Mycosis fungoides; Sézary Syndrome

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