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THE JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS

Rivista di Medicina, Traumatologia e Psicologia dello Sport


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ORIGINAL ARTICLE  EPIDEMIOLOGY AND CLINICAL MEDICINE


The Journal of Sports Medicine and Physical Fitness 2017 June;57(6):839-49

DOI: 10.23736/S0022-4707.17.06254-5

Copyright © 2015 EDIZIONI MINERVA MEDICA

lingua: Inglese

Metabolomic profiles investigation on athletes’ urine 35 minutes after an 800-meter race

Tao SUN, Ying WU, Xueping WU, Haifeng MA

Shanghai University of Sport, Shanghai, China


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BACKGROUND: The aim of this study was to identify possible biological pathways of the metabolite profile changes in athletes’ urine samples before and after 800-m runs.
METHODS: We used an NMR-based metabolomics analysis to evaluate the metabolite profile changes in 19 young male athletes’ urine samples after 800-m runs and provide an overall picture of its impact. Various multivariate data analysis methods, including principal component analysis (PCA), partial least squares-discrimination analysis (PLS-DA), and orthogonal projection of latent-structure-discrimination analysis (OPLS-DA) were applied to analyze the NMR data and thus identify possible correlations between the metabolite profile changes and the alterations in biological pathways.
RESULTS: The potential biological mechanism of an 800-m race was finally elucidated based on the multivariate statistical analysis results. The levels of blood lactate (Lac), 2-hydroxyisovalerate (2HIV), leucine, 2-hydroxyisobutyrate (2HIB), alanine, N-acetyl-glucoprotein, pyruvate, creatinine, fumarate, inosine (Ino) and hypoxanthine (Hyx) were up-regulated in the post samples, whereas the levels of certain metabolites, including 3-hydroxyisovalerate, citrate, taurine, glycine and formate were down-regulated in the postsamples.
CONCLUSIONS: Our study provides novel insights into the 800-m race metabolic characteristic. Separation of pre- from postexercise samples was related to the Krebs cycle, Cori cycle, Cahill cycle, HIFs and ROS. Besides the Lac change, the increased concentrations of Ino, 2HIV concentrations in the postexercise urine samples represent potential indices which indicate the high percent of glycolysis during the 800-m run. The increase of concentrations of Hyx, 2HIB may indicated oxidative stress with concomitant ROS generation in the athletes’ bodies during the 800-m race.


KEY WORDS: Exercise - Metabolomics - Nuclear magnetic resonance, biomolecular - Glycolysis

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Publication History

Issue published online: May 10, 2017
Article first published online: December 23, 2015
Manuscript accepted: December 15, 2015
Manuscript received: October 28, 2015

Per citare questo articolo

Sun T, Wu Y, Wu X, Ma H. Metabolomic profiles investigation on athletes’ urine 35 minutes after an 800-m race. J Sports Med Phys Fitness 2017;57:839-49. DOI: 10.23736/S0022-4707.17.06254-5

Corresponding author e-mail

mahf70@163.com