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Original articles  BODY COMPOSITION, SPORT NUTRITION AND SUPPLEMENTATION (ergogenics)


The Journal of Sports Medicine and Physical Fitness 2004 Settembre;44(3):281-7

lingua: Inglese

The effect of chronic and acute exercise on thymocyte apoptosis and necrosis in ovariectomized mice given dietary genistein

Hoffman-Goetz L., Duerrstein L.

Depart­ment of ­Health ­Studies and Geron­tology Uni­ver­sity of ­Waterloo, ­Waterloo, ON, ­Canada


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Aim. At men­o­pause, ­many ­women con­sume phy­toes­tro­gens ­instead of begin­ning hor­mone replace­ment ­therapy. ­Many ­also ­start exer­cise pro­grams for ­health ­benefìts. Genis­tein, a soy iso­fla­vone ­with ­estrogen-­like prop­er­ties, ­induces lym­pho­cyte apop­tosis in ­vitro. ­Aerobic exer­cise ­also ­induces apop­tosis in lym­phoid ­cells. The ­present ­study was ­designed to deter­mine the ­effect of ­chronic (­wheel run­ning, WR) and ­acute (tread­mill) exer­cise on in ­vivo apop­tosis of thy­moc­ytes ­using an ­animal ­model of men­o­pause ­with sup­ple­men­ta­tion ­from die­tary genis­tein.
­Methods. ­Using a ran­dom­ized ­design, 99 ova­riec­to­mized B6D2F1 ­mice ­were fed 250 (GEN) or 0 (C) ppm genis­tein and ­given con­cur­rent exer­cise (vol­un­tary ­wheel run­ning-WR; or WR fol­lowed by a ­bout of ­high inten­sity tread­mill run­ning-WR+­TREAD) or ­remained sed­en­tary (SED). ­After 21 ­days, ­mice ­were ­sacri­ficed for meas­ure­ments of ­body ­weights, ­tissue ­weights, thy­mo­cyte apop­tosis and ­necrosis by ­annexin-V ­FITC and pro­pidium ­iodide ­staining and ­flow cytom­etry, DNA frag­men­ta­tion by ­ELISA, and ­plasma ­estrogen con­cen­tra­tions by RIA.
­Results. WR+­TREAD ­mice had ­lower per­cent­ages of ­viable and ­higher per­cent­ages of ap­op­totic and ­necrotic ­cells ­from ­thymus com­pared ­with SED or WR con­di­tions (p<0.001). WR ­resulted in ­greater DNA frag­men­ta­tion in ­thymus ­cell ­lysates ­than in sam­ples ­from SED ­mice (p<0.005). ­There ­were no dif­fer­ences in thy­mo­cyte apop­tosis or DNA frag­men­ta­tion ­between GEN and C ­mice, ­either inde­pen­dently or inter­ac­tively ­with exer­cise. GEN ­mice ­tended to ­have ­greater ­wheel run­ning acti­vit­y ­than C ­mice (0.05Con­clu­sion. ­These ­fìndings sug­gest ­that 1) ­intense ­aerobic exer­cise ­increases the per­centage of ­early apop­totic ­cells in the ­thymus and 2) die­tary genis­tein is asso­ciated ­with a ­small ­increase in vol­un­tary ­wheel run­ning ­activity in ova­riec­to­mized ­mice.

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