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Indexed/Abstracted in: Chemical Abstracts, CINAHL, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
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Child R. B., Wilkinson D. M., Fallowfield J. L.
Exercise Physiology Research Group, Centre for Sport Sciences, University College Chichester, College Lane, Chichester, UK
Background. This study tested the hypothesis that the ability to scavenge free radicals in serum was compromised in trained runners.
Methods. Experimental design: peak V.O2, the ability to scavenge free radicals in serum and the plasma concentration of malondialdehyde (MDA) were assessed in 18 male runners. Participants: subject characteristics (mean±SEM) were height 1.77±0.01 m, mass 71.4±1.2 kg, age 31±1 years and weekly training distance 45±5 km.week-1. Measures: venous blood samples were collected at rest. Serum total antioxidant capacity (TAC) was determined using a chemiluminescent technique. This involved the oxidation of luminol, in a reaction catalysed by horseradish peroxidase. Serum antioxidant protection was quantified relative to a soluble vitamin E analogue (Trolox) and expressed as Trolox equivalents (Trolox Eq.). MDA was determined using a highly specific assay, using HPLC with fluorimetric detection. Peak V.O2 was determined from expired gas measurements collected during an incremental running test on a motorised treadmill. Data were analysed using Pearson correlations.
Results. Serum TAC was 500±26 µmol Trolox Eq.l-1, with
a plasma MDA concentration of 1.5±0.1 mmol.l-1 and
serum urate concentration of 274±12 mmol.l-1. Peak V.O2 was 63±1 ml.kg-1.min-1. Significant correlations were observed between peak V.O2 and serum TAC (r=0.365, p<0.05); peak V.O2 and serum urate (r=0.463, p<0.05) and serum urate and serum TAC (r=0.807, p<0.001). Plasma MDA and serum TAC were not significantly correlated (r=0.026, p>0.05).
Conclusions. These data demonstrate that the ability to quench free radicals in serum is increased in relation to the maximum ability to consume oxygen, however this response does not appear to provide any additional protection against peroxidative damage at rest.