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The Journal of Sports Medicine and Physical Fitness 1999 December;39(4):269-74

Copyright © 2000 EDIZIONI MINERVA MEDICA

lingua: Inglese

In ­vitro apop­tosis of lym­pho­cytes ­after expo­sure to ­levels of cor­ti­cos­te­rone ­observed fol­lowing sub­max­imal exer­cise

Hoffman-Goetz L., Zajchowski S.

Depart­ment of ­Health ­Studies and Geron­tology, Uni­ver­sity of ­Waterloo, Waterloo, ­Ontario, ­Canada


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Back­ground. To eval­uate the in ­vitro ­effects of cor­ti­cos­te­rone, equiv­a­lent to ­blood ­levels meas­ured ­after a ­single sub­max­imal tread­mill exer­cise, on apop­tosis and ­necrosis of ­mouse ­thymic and periph­eral lym­pho­cytes.
­Methods. Experi­mental ­design: anal­ysis of var­i­ance ­with inde­pen­dent fac­tors of ­time (0, 90, 210 min­utes of incu­ba­tion) and con­cen­tra­tion of cor­ti­cos­te­rone (0, 150, 250, 450, 850 ng/ml). Meas­ures: per­centage of apop­totic, ­necrotic, and ­viable thy­moc­ytes and sple­noc­ytes deter­mined by ­flow cytom­etry ­using ­Annexin V-­FITC anti­body and pro­pidium ­iodide.
­Results. ­There was a sig­nif­i­cantly ­higher % of apop­totic thy­moc­ytes at 210 min at the ­higher cor­ti­cos­te­rone con­cen­tra­tions but not in % of apop­totic sple­noc­ytes at the ­same ­time ­point. For ­both lym­phoid pop­u­la­tions, cor­ti­cos­te­rone incu­ba­tion was asso­ciated ­with a ­higher % of ­necrotic ­cells at 210 min­utes but not at 0 or 90 min­utes. For thy­moc­ytes, the inter­ac­tion (­time x con­cen­tra­tion) was sig­nif­i­cant, ­with ­greater % ­necrosis ­observed at the ­lower (150 and 250 ng/ml) con­cen­tra­tions of cor­ti­cos­te­rone.
Con­clu­sions. The ­data sug­gest ­that in ­vitro expo­sure to cor­ti­cos­te­rone, at phys­io­log­ical con­cen­tra­tions (≤450 ng/ml) ­observed ­after a mod­erate exer­cise ­stress, ­induces apop­tosis in thy­moc­ytes and ­necrosis in ­both thy­moc­ytes and sple­noc­ytes. The impli­ca­tions for exer­cise-medi­ated glu­co­cor­ti­coid reg­u­la­tion of ­immune func­tion ­remain to be inves­ti­gated.

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