Home > Riviste > Panminerva Medica > Fascicoli precedenti > Panminerva Medica 2014 September;56(3) > Panminerva Medica 2014 September;56(3):211-20

ULTIMO FASCICOLO
 

ARTICLE TOOLS

Estratti

PANMINERVA MEDICA

Rivista di Medicina Interna


Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,698


eTOC

 

ORIGINAL ARTICLES  


Panminerva Medica 2014 September;56(3):211-20

Copyright © 2014 EDIZIONI MINERVA MEDICA

lingua: Inglese

Triptolide sensitizes liver cancer cell lines to chemotherapy in vitro and in vivo

Li Y., Hu S.

Department of General Surgery, Qilu Hospital, Shandong University, Jinan, China


PDF  


AIM: Triptolide is a diterpenoid epoxide isolated from the herb Tripterygium wilfordii, which has long been used as a natural medicine in China for many diseases including cancer. However, its effect on liver cancer has not yet been evaluated. The aim of this study is to investigate the effect of triptolide on liver cancer cell lines.
METHODS: The effect of triptolide with cancer chemotherapeutics such as cisplatin and fluorouracil (5-FU) in vitro and in vivo on liver cancer cells has been investigated. Cell viability and protein expression were determined by MTT assay and Western blotting respectively. Aoptosis and reactive oxygen species (ROS) were detected by flow cytometry. The antitumor effects of triptolide alone or in combination were also measured in transplantation tumor models.
RESULTS: The combination of triptolide plus chemotherapeutics (Cisplatin, 5-FU) reduced cancer cell viability and enhanced apoptosis compared with single treatment in vitro. Furthermore, cells treated with triptolide plus chemotherapeutics exhibited marked production of intracellular ROS and caspase-3 activity compared with single treatment, which paralleled the incidence of apoptosis. Triptolide and chemotherapeutics treatment also induced Bax expression, and inhibited Bcl-2 expression. Triptolide also boosted the anticancerous effects of cisplatin and 5-FU in nude mice compared with cisplatin or 5-FU alon treated tumors.
CONCLUSION: Our study demonstrated that triptolide is associated with increased cellular sensitivity of liver cancer cells to cisplatin and 5-FU in vivo and in vitro, and may provide new liver cancer treatment strategies.

inizio pagina

Publication History

Per citare questo articolo

Corresponding author e-mail

pxliyong@163.com