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Panminerva Medica 2002 December;44(4):343-7

lingua: Inglese

Large granular lymphocytes (LGLS) activity in women with spontaneous abortions during the 1st trimester of gestation

Papadopoulos N. 1, Simopoulos C. 2, Karamanidis D. 3, Kotini A. 4, Tamiolakis D. 5

1 Department of Histology-Embryology, Democritus University of Thrace, Alexandroupolis, Greece
2 Department of Experimental Surgery, Democritus University of Thrace, Alexandroupolis, Greece
3 Department of Medical Physics, Democritus University of Thrace, Alexandroupolis, Greece
4 Department of Obstetrics and Gynecology, Regional Hospital of Alexandroupolis, Greece
5 Department of Cytology, Regional Hospital of Alexandroupolis, Greece


Background. Despite ­increasing knowl­edge ­about the ­cell sub­sets ­that ­exist in the ­decidua in ­normal ­early preg­nancy, ­little is ­known ­about the ­decidual ­cell sub­sets in ­women ­with spon­ta­neous preg­nancy ­failure. Decidual ­large gran­ular lym­pho­cytes (LGLs) are the ­most abun­dant lym­phoid ­cell ­type ­found in 1st tri­mester ­maternal ­decidua. The func­tion of LGLs ­remains con­tro­ver­sial ­although ­freshly iso­lated LGLs ­have ­been ­shown to ­exert a ­weak nat­ural ­killer ­cell (NK) ­activity.
Methods. Decidual leu­ko­cytes ­were inves­ti­gated in 15 ­cases of spon­ta­neous abor­tion and 15 ­cases of vol­un­tary mis­car­riage, at 8th, 10th and 12th ges­ta­tional ­week ­using a ­panel of mono­clonal anti­bodies ­against Granzyme B (­GRAN-B), CD2, CD38, CD56, and CD57 anti­gens and the immu­nop­e­rox­i­dase tech­nique. LGLs ­were dem­on­strated ­with the ­phloxine-tar­ta­zine ­stain.
Results. There was a sta­tis­ti­cally sig­nif­i­cant ­increase in the ­number of CD56 and CD57 pos­i­tive ­cells (33.3%) to the advan­tage of spon­ta­neous abor­tions (p<0.0001). No sta­tis­ti­cally sig­nif­i­cant dif­fer­ence was ­found in the ­number of ­GRAN-B, CD2 and CD38 pos­i­tive ­cells in ­normal ­early preg­nancy and in spon­ta­neous abor­tion (­overall ges­ta­tional age p=0.22, 8th ges­ta­tional ­week p=0.18, 10th and 12th ges­ta­tional ­week, p=0.091).
Conclusions. Our find­ings con­firm pre­vious ­studies refer­ring ­that LGLs may ­have a cyto­toxic ­activity sim­ilar to the NK ­activity and sug­gest an ­adverse preg­nancy out­come.

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