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Panminerva Medica 2001 December;43(4):249-55

lingua: Inglese

p53 expression in cultured blood human monocytes infected with mycobacterial strains

Galietti F., Bollo E. *, Cappia S. **, Dondo A. ***, Pregel P. *, Nicali R., Pozzi E.

From the Pulmonology Department Turin University at S. Luigi Gonzaga Hospital Orbassano, Turin, Italy
*Animal Pathology Department University of Turin, Turin, Italy
**Pathology Unit S. Luigi Gonzaga Hospital, Orbassano, Turin, Italy
***Institute for Zooprophylaxis, Turin, Italy


Background. An upreg­u­la­tion of the ­cell-­cycle asso­ciat­ed pro­teins p53 and p21/Waf1/Cip1 ­induced by myco­bac­te­ria was pre­vi­ous­ly report­ed. We ­aimed to eval­u­ate the expres­sion of ­such pro­teins in periph­er­al ­blood ­human mono­cyte cul­tures infect­ed ­with ­strains of dif­fer­ent myco­bac­te­ri­al patho­gens.
Methods. The ­study ­relied on the immu­no­cy­to­chem­i­cal deter­mi­na­tion of p53, p21/Waf1/Cipl, bcl-2 and on the Tunel detec­tion of apop­to­sis in mono­cy­tes pop­u­la­tions cul­tured on ­four-­welled cham­ber ­slides (106 ­cells/­well) infect­ed ­with Mycobacterium tuber­cu­lo­sis, M. ­bovis and M. avi­um for ­four con­sec­u­tive ­days (myco­bac­te­ri­um/mono­cyte ­ratio 10:1). The ­results ­were ­expressed as ­mean val­ues and SD of the per­cent­ag­es of stain­ings record­ed in five ­fields per ­slide.
Results. The sta­tis­ti­cal anal­y­sis ­with Fischer ­test dem­os­trat­ed ­that at ­most sam­pling ­times the p53 and p21/Waf1/Cip1 expres­sion and the apop­to­sis ­index ­were sig­nif­i­cant­ly high­er in M. tuber­cu­lo­sis infect­ed cul­tures ­than in con­trols (p<0.05). The M. ­bovis relat­ed pic­ture ­diverged ­from the pre­vi­ous one for a low­er p53 expres­sion (p<0.05) at all sam­pling ­times. The M. avi­um infect­ed cul­ture val­ues did not ­diverge sig­nif­i­cant­ly ­from the con­trols.
Conclusions. The p53 and p21/Wafl/Cipl upreg­u­la­tion is com­pat­ible ­with ­both ­host ­defense strat­e­gies and patho­gen strat­e­gies (safe­guard of intra­cel­lu­lar sanc­tu­ar­ies). The dis­crep­an­cies ­among dif­fer­ent cul­tures sug­gest a ­direct rela­tion­ship ­between p53 acti­va­tion and myco­bac­te­ri­al abil­ity to ­enter ­host ­cells.

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