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THE QUARTERLY JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
Rivista di Medicina Nucleare e Imaging Molecolare
A Journal on Nuclear Medicine and Molecular Imaging
Affiliated to the and to the International Research Group of Immunoscintigraphy
Indexed/Abstracted in: Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index (SciSearch), Scopus
Impact Factor 2,413
The Quarterly Journal of Nuclear Medicine 2003 Giugno;47(2):139-44
Radioimmuno targetting technetium 99mlabeled anti-epidermal growth factor receptor monoclonal antibody in experimental tumor models
Meenakshi A., Ganesh V., Suresh Kumar R., Siva Kumar N.
Department of Biochemical Oncology Cancer Institute (WIA) Regional Center for Cancer Research and Treatment of Government of India Adyar, Chennai, India
Aim. Monoclonal antibodies (MAb) directed at the extra cellular domain (ECD) of epidermal growth factor receptor (EGFR) offer a promising strategy for diagnosis and therapy of cancers that over-express EGFR. Radiolabelled MAbs against cell surface antigens have improved in vivo tumor diagnosis and treatment. EGFR over-expression has been reported in a wide range of carcinomas especially of the head and neck, breast, etc., and is associated with poor prognosis and resistance to therapy. CIBCNSH3 is a murine MAb generated to the ECD of EGFR in our laboratory and has been extensively characterized and has proven antitumor activity. The tumor targeting potential of 99mTc labelled CIBCNSH3 in an experimental tumor model is discussed in this paper.
Methods. A431, an epidermoid carcinoma cell line with overexpression of EGFR, SUDHLH, a lymphoma cell line was used to induce xenografts in inbred adult female BALB/C mice and used for the study. A reduction mediated method of 99mTc labelling was adopted to label the MAb. Scintiscan pictures were taken at different time intervals after i.v. administration of the 99mTc labelled MAb using a gamma camera and results were correlated with those of biodistribution studies.
Results. Immunoscan pictures taken at different time periods showed high uptake of the radioimmunoconjugate by the tumor providing clear tumor images and no uptake in control animals with lymphoma xenografts. Results of scan pictures correlated well with the biodistribution studies.
Conclusion. The radioimmunoconjugate 99mTc-CIBCNSH3 appears to be a promising tool in identifying any early recurrence and micro-metastasis of lesions that overexpress EGFR.