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Indexed/Abstracted in: e-psyche, EMBASE, PubMed/MEDLINE, Neuroscience Citation Index, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,651
Online ISSN 1827-1855
Huang W. 1, Chen M. 2, Zhang Y. 2, Zhao P. 3, Yao G. 4, Zhou H. 2
1 Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, China;
2 Department of Neurology, Daping Hospital, Third Military Medical University, Chongqing, China;
3 Department of Medical Education, Daping Hospital, Third Military Medical University, Chongqing, China;
4 Department of Neurology, The First Affiliated Hospital of General Hospital of PLA, Beijing, China
AIM: The aim of the study was to determine the relationship between Apo Eε4 genotype and senile dementia (SD) by analyzing the Apo E allelic frequency distributions among the elderly Han Chinese population.
METHODS: For this purpose, a total of 316 Chongqing residents, aged ≥60yrs, were classified as SD or control groups following the criteria of National Institute of Neurological and Communicative Disorders and Stroke-Alzheimer’s Disease and Related Disorders Association. Genomic DNA was isolated from the peripheral blood lymphocytes and exon 4 of the ApoE gene with polymorphism sites was amplified by PCR and genotypes determined by restriction fragment length polymorphism (RFLP).
RESULTS: We found that the most prevalent genotype was Apo Eε3/3, followed in order by Apo Eε3/4 and Apo Eε2/2. The estimated ApoE allelic frequencies in individuals with SD were 0.095, 0.560, and 0.345 for ε2, ε3, and ε4, respectively. In controls, the corresponding Apo E allelic frequencies were 0.146, 0.699, and 0.155. The percentage of ε4 allele carriers in SD group was significantly higher than that in control group (P<0.01); while those of ε2 and ε3 genotypes were lower in SD group as compared with control group.
CONCLUSION: We concluded that in the elderly Han Chinese residents of Chongqing aged 60 years and over, Apo Eε3/3 and Apo Eε2/2 were the most and least prevalent genotypes, respectively. Further, based on strong linkage, Apo Eε4 allele might be a significant risk factor for the development of senile dementia.