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Journal of Neurosurgical Sciences 2001 March;45(1):1-6

lingua: Inglese

Inverse cor­re­la­tion of TRF1 expres­sion and ­cell pro­life­ra­tion in ­human pri­mary intra­cra­ni­al ­tumors

De Divitiis O., La Torre D.

Department of Neurosurgery, Polyclinic, University of Messina, Messina, Italy

Background. The tel­o­mer­ic-­repeat bind­ing fac­tor (TRF1) par­tic­i­pates in a phys­io­log­i­cal homeo­stat­ic mech­a­nism con­trol­ling cel­lu­lar pro­life­ra­tive poten­tial. TRF1 is ­involved in a neg­a­tive feed­back mech­a­nism ­that ­allows tel­o­mere short­en­ing by inhib­it­ing the activ­ity of telom­e­rase. Down-reg­u­la­tion of TRF1 expres­sion ­results in tel­o­mere elon­ga­tion and may be ­involved in ­cell immor­tal­iza­tion. The ­goal of the ­present ­study was to deter­mine wheth­er rou­tine immu­no­his­to­chem­i­cal (IHC) tech­niques can char­ac­ter­ize TRF1 expres­sion in dif­fer­ent ­human ­brain ­tumor spec­i­mens and wheth­er it cor­re­lates ­with oth­er indi­ces of ­brain ­tumor’s pro­life­ra­tive poten­tial.
Methods. A ­cohort of 20 ­flash-fro­zen sur­gi­cal spec­i­mens [14 menin­gio­mas and 6 ana­plas­tic astro­cy­to­mas (AA)] ­were eval­u­at­ed for TRF1 expres­sion. Results of par­allel inves­ti­ga­tions of ­tumor’s pro­life­ra­tive indi­ces as ­assessed by Ki67 label­ing ­index (LI) deter­mi­na­tions ­were ­cross-cor­re­lat­ed ­with TRF1 expres­sion ­results and his­to­type.
Results. We dem­on­strat­ed var­i­able lev­els of TRF1 expres­sion in 12 out of 14 (87.5%) menin­gio­ma sam­ples. By con­trast, we detect­ed no expres­sion of TRF1 in tis­sue sam­ples ­from AA (p=0.008). The Ki67 LI was high­er in AA ­than in menin­gio­ma sam­ples (15.21±9.34 vs 26.6±13.89, p=0.044). Statistical anal­y­sis ­revealed a sig­nif­i­cant ­inverse cor­re­la­tion ­between TRF1 expres­sion, his­to­type, and LI (χ2=14.1; p=0.0008).
Conclusions. We dem­on­strat­ed for the ­first ­time ­that rou­tine IHC tech­niques are ­capable to iden­ti­fy­ing TRF1 expres­sion in intra­cra­ni­al ­tumors. The ­results sug­gest ­that TRF1 is het­er­o­ge­ne­ous­ly ­expressed in menin­gio­mas, and ­absent in AA. The TRF1 stat­us in intra­cra­ni­al ­tumors ­might be of prog­nos­tic val­ue and pos­sibly rep­re­sent a poten­tial appli­ca­tion for bio­log­i­cal­ly tar­get­ed ther­a­peu­tic strat­e­gies.

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