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Montree TOSO 1, Somying PATNTIRAPONG 1, Wanida JANVIKUL 2, Weerachai SINGHATANADGIT 1,
1 Faculty of Dentistry, Thammasat University, Pathumthani, Thailand; 2 Biomedical Engineering Research Unit, National Metal and Materials Technology Center, Pathumthani, Thailand
BACKGROUND: Poly(butylene succinate) (PBSu) and PBSu/β-tricalcium phosphate (TCP) composites are biocompatible and good candidates as bone graft materials. However, little is known about the responses of T lymphocytes to these biomaterials, which play an important role in the success of bone grafting.
METHODS: Activated T lymphocytes were seeded onto 32 mm diameter films (PBSu, PBSu/TCP8020 and PBSu/TCP6040) that have previously placed in 6-well culture plates for 8 h, 24 h and 72 h. A plastic-well culture plate was used as a control surface. The effects of PBSu-based biomaterials on T lymphocytes were examined by the using flow cytometry and reverse transcription-polymerase chain reaction.
RESULTS: These biomaterials were non-toxic to T lymphocytes, allowing their normal DNA synthesis and activation. All materials induced only transient activation of T lymphocytes, which existed no longer than 72 h. Proportions of four main CD4/CD8 T lymphocyte subpopulations were not affected by these biomaterials. Moreover, PBSu and PBSu/TCP significantly suppressed the expression of IL-1β and IL-6 genes by 15%-35% and 21%-26%, respectively. In contrast, a PBSu/TCP composite (at PBSu:TCP = 60:40) significantly stimulated the expression of IL-10 and IL-13 genes by 17% and 19%, respectively.
CONCLUSIONS: PBSu and PBSu/TCP composites were non-toxic to T lymphocytes and did not induce unfavorable responses of T lymphocytes. The tested biomaterials down-regulated key proinflammatory cytokine genes and up-regulated anti-inflammatory cytokine genes in T lymphocytes. These suggest that the biomaterials studied are good candidates as bone graft materials.