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FASCICOLI E ARTICOLI   I PIÙ LETTI   eTOC

ULTIMO FASCICOLOMINERVA PNEUMOLOGICA

Rivista sulle Malattie dell’Apparato Respiratorio


Official Journal of the Italian Society of Thoracic Endoscopy
Indexed/Abstracted in: EMBASE, Scopus

 

Minerva Pneumologica 2016 Settembre;55(3):31-6

 ORIGINAL ARTICLES

Expression of two essential mRNA biomarker in the peripheral blood as possible biomarkers for diagnosis of non‑small cell lung carcinoma

Naghmeh BAHRAMI 1, 2, Mina GHOLAMI 3, Hamid R. JAMAATI 4, Abdolreza MOHAMADNIA 5, 6, Hossein DARGAHI 7, Mehdi KAZEMPOUR DIZAJI 8, Adnan KHOSRAVI 9, Jalal HESHMAT NIA 10, Parisa VAHABI 11, Naeim BAHRAMI 12, 13

1 Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; 2 Oral and Maxillofacial Surgery Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran; 3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; 4 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRI TLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5 Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRI TLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 6 Biotechnology Department, Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 7 Allied Medicine, Health Information Management Research Center, Tehran University of Medical Sciences, Tehran, Iran; 8 Biostatistics Department, Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRI TLD), Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 9 Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRI TLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 10 National Research Institute of Tuberculosis and Lung Diseases (NRI TLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 11 School of Nursing, Tehran University of Medical Sciences, Tehran, Iran; 12 Department of Biomedical Engineering, Virginia Tech‑Wake Forest University, Blacksburg, VA, USA; 13 Wake Forest University, School of Medicine, Winston‑Salem, NC, USA

BACKGROUND: Despite medical advances, lung cancer often remains undiagnosed until advanced stages mainly due to the lack of specific tumor markers. This study sought to assess vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA ) mRNA biomarkers in the peripheral blood of non‑small cell lung carcinoma (NSCLC ) patients for possible use in diagnosis and early screening of these patients.
METHODS: Peripheral blood samples were drawn of 30 NSCLC patients and 30 healthy controls; RNA was extracted and of each RNA sample, cDNA was synthesized in triplicate. Real‑time quantitative reverse‑transcription polymerase chain reaction (RT‑qPCR ) was used to quantify VEG F and CEA mRNA biomarkers.
RESULTS: Of all patients, 86.6% (26 out of 30) were positive for VEG F mRNA; this rate was 13.3% (4 out of 30) in healthy controls. Of all patients, 90% (27 out of 30) were positive for CEA mRNA; this rate was 33.3% (10 out of 30) in healthy controls.
CONCLUSIONS: Based on the results, VEGF and CEA mRNA seem to be beneficial biomarkers for the diagnosis of NSCLC using peripheral blood samples. However, simultaneous use of several molecular biomarkers is proposed to achieve more reliable results.

lingua: Inglese


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