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Rivista sulle Malattie dell’Apparato Respiratorio
Official Journal of the Italian Society of Thoracic Endoscopy
Indexed/Abstracted in: EMBASE, Scopus, Emerging Sources Citation Index
Minerva Pneumologica 2016 March;55(1):15-22
DNA damage and ıts association with oxidative and antioxidative measurements in children with non cystic fibrosis bronchiectasis
Dost ZEYREK 1, Hasan KAPAKLI 2, Alpay CAKMAK 2, Abdurrahim KOCYİGİT 3
1 Division of Pediatric Pulmonology, Department of Pediatrics, Harran University School of Medicine, Şanlıurfa, Turkey; 2 Department of Pediatrics, Harran University School of Medicine, Şanlıurfa, Turkey; 3 Department of Biochemistry, Bezmialem University School of Medicine, Istanbul, Turkey
BACKGROUND: Bronchiectasis is a chronic inflammatory pulmonary disease. Due to increased inflamation, oxidative stress is present in bronchiectasis. In this study, we aimed to find DNA damage and its association with total antioxidant status (TAS), total oxidant status (TOS) and Oxidative Stress Index (OSI) in children with non-cystic fibrosis bronchiectasis.
METHODS: Forty-five patients with non-cystic fibrosis bronchiectasis with mean age of 10.4±3.27 years and 36 healthy children with mean age of 9.36±3.65 were included in this study. The control group of the same age and socio-demographic conditions compatible with the patient group was selected from children. Mononuclear leukocyte DNA damage was analysed by alkaline single cell electrophoresis assay (Comet Assay). Plasma TOS and TAS levels were measured by Erel O. method and OSI values were calculated.
RESULTS: In the patients with non-cystic fibrosis bronchiectasis, plasma TOS, OSI leves and mononuclear leukocyte DNA damage were significantly higher than the control group (respectively, P<0.01, P<0.001, P<0.001). The average TAS in patients with non-cystic fibrosis bronchiectasis group were significantly lower than the control group (P<0.001). There is a positive correlation between the oxidative stress and DNA damage.
CONCLUSIONS: We report that oxidative stress and DNA damage levels are higher in the patient with non-cystic fibrosis bronchiectasis. This finding shows that there is a connection between the increase in oxidative stress level and DNA damage in children with non-CF bronchiectasis